BE/GEN-EN: Functional Genomics: Interactions Among Ecology, Population Biology and Gene Expression

BE/GEN-EN：功能基因组学：生态学、群体生物学和基因表达之间的相互作用

• 批准号: 0308777
• 负责人: Douglas Crawford
• 金额: $ 199.87万
• 依托单位: University of Miami
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-02-01 至 2008-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0308777&HistoricalAwards=false
• 关键词: GEN; EN; Functional; Genomics; Interactions

ABSTRACTThe fish Fundulus heteroclitus is widely distributed in estuaries along the eastern seacoast of North America. This species is found in areas where there is considerable variation in habitat spatial heterogeneity (i.e., landscape complexity) and in a region characterized by one of the world's steepest thermal clines: approximately 1.0 degree centigrade per degree of latitude. The goals of this project are to determinehow landscape complexity within a local region and along the steep thermal cline affect genome-wide patterns of gene expression. Landscape structure is hypothesized to affect population size and genetic structure, and individual growth rates, longevity, and migration. These parameters in turn alter the process of natural selection and thus affect an organism's ability to adapt to local conditions. This project will measure theseecological and population variables and explore their relationships to patterns of gene expression. Genetic crosses of field-collected populations, and laboratory acclimation of their progeny, will determine if physiological induction or heritable genetic differences are responsible for the differences in gene expression. Genome-wide patterns of mRNA expression will be quantified with F. heteroclitus microarrays. Because variation in messenger RNA must be translated into changes in protein concentration to produce a phenotypic change, microarray experiments will be complemented by high-throughput analyses of protein expression (proteomics). If variation in gene expression is one of the most important adaptive mechanisms, this project will detect significant differences in gene expression associated with differences in landscape and thermal ecology. This research will determine how ecological factors operating at diverse geographic scales affect the population biology and gene expression of F. heteroclitus.

摘要，鱼类杂质在北美东部海岸沿线的河口广泛分布。该物种在栖息地空间异质性（即景观复杂性）和以世界上最陡峭的热层之一为特征的区域中发现了相当大的差异：每纬度每度约1.0度。该项目的目标是确定局部区域内和陡峭的热层内的景观复杂性影响基因表达的全基因组模式。假设景观结构会影响人口规模和遗传结构，以及个体的增长率，寿命和迁移。这些参数反过来改变了自然选择的过程，从而影响了生物体适应局部条件的能力。该项目将衡量这些生态和人口变量，并探索它们与基因表达模式的关系。现场收集的种群的遗传杂交以及对后代的实验室适应，将确定生理诱导或可遗传的遗传差异是否导致基因表达的差异。 mRNA表达的全基因组模式将用F. heteroclitus微阵列定量。由于信使RNA的变化必须转化为蛋白质浓度的变化以产生表型变化，因此通过对蛋白质表达（蛋白质组学）的高通量分析来补充微阵列实验。如果基因表达的变化是最重要的自适应机制之一，则该项目将检测到与景观和热生态学差异相关的基因表达的显着差异。这项研究将确定在各种地理量表上工作的生态因素如何影响杂孢菌的种群生物学和基因表达。