Evolutionary Analyses of the Molecular Mechanisms Regulating Ldh-B Expression

Ldh-B表达调控分子机制的进化分析

基本信息

批准号：
0355189
负责人：
Douglas Crawford
金额：
$ 7.03万
依托单位：
University of Miami
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2003
资助国家：
美国
起止时间：
2003-06-01 至 2005-02-28
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=0355189&HistoricalAwards=false
关键词：
Evolutionary Analyses Molecular Mechanisms Regulating

项目摘要

Many researchers have suggested that variation in enzyme expression is an important mechanism for adaptive change, yet few studies address the molecular mechanisms responsible for these changes. Closely related species of the killifish, Fundulus, provide a system to investigate these mechanisms and thus address the molecular changes responsible for the adaptive variation in the glycolytic enzyme, lactate dehydrogenase B (LDH-B) expression. Populations among these species have independently evolved in different thermal environments and show adaptive changes in the levels of LDH enzyme. One can investigate the DNA sequences that control enzyme expression among these populations and, by applying the appropriate evolutionary analyses, distinguish between random DNA sequence variations and DNA sequence variations that has evolved by natural selection. The functional variation in DNA sequences that affect enzyme expression among these closely related taxa also will be investigated. By examining both the evolutionary and functional significance of DNA sequence variations, this research seeks to clarify the molecular mechanisms that produce adaptive changes in enzyme expression. The analyses among closely related species are more likely to identify the specific molecular changes responsible for adaptive variation than studies that focus on single model organisms. Thus, these studies provide unique information and a persuasive perspective concerning DNA sequence variation that could be critical for the health or performance of humans and other animals.

许多研究人员表明，酶表达的变化是适应性变化的重要机制，但是很少有研究解决了导致这些变化的分子机制。小杀虫的密切相关的物种，提供了一个研究这些机制的系统，因此解决了导致糖酵解酶自适应变化的分子变化，乳酸脱氢酶B（LDH-B）表达。这些物种中的种群在不同的热环境中独立发展，并显示出LDH酶水平的适应性变化。可以研究这些人群中控制酶表达的DNA序列，并通过应用适当的进化分析来区分随机选择而通过自然选择演变而来的随机DNA序列变化和DNA序列变化。还将研究影响这些密切相关的类群中酶表达的DNA序列的功能变化。通过检查DNA序列变化的进化和功能意义，该研究旨在阐明在酶表达中产生适应性变化的分子机制。与关注单个模型生物的研究相比，密切相关物种之间的分析更有可能确定导致适应性变化的特定分子变化。因此，这些研究提供了有关DNA序列变化的独特信息和有说服力的观点，这对于人类和其他动物的健康或表现至关重要。