BE/GEN-EN: Genome Sequence and Functional Analysis of an Uncultured Bacterium

BE/GEN-EN：未培养细菌的基因组序列和功能分析

• 批准号: 0221798
• 负责人: Steven Gill
• 金额: --
• 依托单位: Institute for Genomic Research
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-10-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0221798&HistoricalAwards=false
• 关键词: GEN; EN; Genome; Sequence; Functional

ABSTRACTAt 80 by 600 microns, Epulopiscium is the largest heterotrophic bacterium that has been identified to date. It also has the unusual ability to produce multiple live offspring internally. This project takes an aggressive approach to increase our understanding of the biology of this organism by determining the complete genome sequence, and identifying the unique features of Epulopiscium that have allowed these exceptional bacteria to attain cellular sizes that are orders of magnitude larger than most bacteria. These analyses will identify genomic and cellular modifications that may have been employed in the progenitor of all eukaryotes as a first step toward the development of the contemporary eukaryotic cell. Comparative analyses with the complete genome sequences of endospore-forming bacteria will identify sporulation gene homologues in the Epulopiscium genome that can serve as targets of future analyses to determine the role of these genes in intracellular offspring production. Finally, this project will generate a physiological profile of Epulopiscium sp. based on the complete genome sequence. This component will address how the normal environment of this species (the guts of surgeonfish) may have enabled the evolution of these life-style novelties, and serve as a framework for developing culture media to grow Epulopiscium in the laboratory so that more detailed experiments on this species can be conducted. Collaborations, discussions, and data exchange with both domestic and international groups that are studying Epulopiscium biology, spore-forming bacteria, and extremely large bacteria will be encouraged. Training and educational workshops will be held at The Institute for Genomic Research (TIGR) in Rockville, Maryland for these biologists. The project team will make every attempt to ensure that underrepresented groups are included in the students and post-doctoral candidates who will be involved for the duration of the proposed study. Sequence and microarray data will be continuously posted and updated on our public web site (www.tigr.org).

epulopiscium Abstractat 80 x 600微米是迄今已鉴定出的最大的异养细菌。它还具有在内部产生多个生物后代的异常能力。该项目采用一种积极的方法来通过确定完整的基因组序列来增强我们对这种生物体的生物学的理解，并确定epulopiscium的独特特征，这些特征使这些特殊细菌获得了比大多数细菌大的数量级的细胞大小。这些分析将确定所有真核生物祖细胞可能使用的基因组和细胞修饰是迈向当代真核生物细胞发展的第一步。与内生孢子细菌的完整基因组序列进行比较分析将确定e骨基因组中的孢子形成基因同源物，这些基因组可以用作未来分析的靶标，以确定这些基因在细胞内后代产生产生中的作用。最后，该项目将产生e骨sp的生理特征。基于完整的基因组序列。该组成部分将解决该物种的正常环境（外科医生的肠道）如何使这些生活方式的新颖性的演变，并作为开发培养基在实验室中生长epulopiscium的框架，以便可以对该物种进行更详细的实验。将鼓励与研究e骨生物学，形成孢子的细菌以及极大的细菌的国内和国际团体的合作，讨论和数据交流。这些生物学家将在马里兰州罗克维尔的基因组研究研究所（TIGR）举行培训和教育研讨会。项目团队将尽一切努力确保将代表性不足的群体包括在学生和博士后候选人中，他们将在拟议的研究期间参与其中。序列和微阵列数据将在我们的公共网站（www.tigr.org）上连续发布和更新。