Microbial Genome Sequencing: Sequencing of Plasmids from the Bacillus Cereus Group-Mapping the Evolution of B. Anthracis Virulence Plasmids px01 and px02

微生物基因组测序：蜡状芽孢杆菌组质粒的测序 - 绘制炭疽芽孢杆菌毒力质粒 px01 和 px02 的进化图谱

• 批准号: 0242162
• 负责人: Steven Gill
• 金额: $ 99.87万
• 依托单位: Institute for Genomic Research
• 依托单位国家: 美国
• 项目类别: Continuing grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-10-01 至 2005-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0242162&HistoricalAwards=false
• 关键词: Microbial; Genome; Sequencing; Plasmids; Bacillus

The aim of this project is to make a comprehensive survey of complete sequences of large plasmids from the Bacillus cereus group of bacteria, which are ubiquitous constituents of natural soil environments, endosymbionts of insects, and animal pathogens. Perhaps the most well known member of the group is the organism, B. anthracis. In a theme common to the B. cereus group, key virulence genes, encoding lethal toxin and poly-glutamic acid capsule, that are responsible for the unique pathogenicity profile of B. anthracis are found on large plasmids (pXO1 and pXO2). Because plasmids play a major role in the biology of anthrax, a survey of large plasmids in up to 24 B. cereus group strains is proposed to answer specific questions about plasmids with and without known homologies to pXO1 and pXO2. These questions include: can evolutionary events that led to the creation of pXO1 and pXO2 be reconstructed and can sources for some of the genes on these plasmids be found in other B. cereus group organisms? To what extent does gene exchange between replicons occur and what are the mechanisms? Which pXO genes are unique in B. anthracis? Plasmids will be isolated from pulsed-field gels of genomic DNA and sequenced by whole-genome shotgun methods to produce finished, high-quality sequence. This project will significantly add to knowledge of how extrachromsomal elements influence key phenotypes of these important microorganisms. This is a Genome Sequencing Award funded through a collaborative program between the National Science Foundation and the Department of Agriculture.

该项目的目的是对蜡细菌杆菌的完整序列进行全面调查，这些细菌是天然土壤环境，昆虫的内共生和动物病原体的无处不在的成分。也许该小组最著名的成员是生物体炭疽菌。 在蜡状芽孢杆菌组共有的主题中，主要的毒力基因编码致命的毒素和多谷氨酸胶囊，这些基因在大质粒上发现了炭疽芽孢杆菌的独特致病性特征（PXO1和PXO2）。 由于质粒在炭疽的生物学中起主要作用，因此提出了对蜡状组菌株的大型质粒的调查，以回答有关有和没有已知同源性PXO1和PXO2的质粒的特定问题。 这些问题包括：可以重建导致PXO1和PXO2创建的进化事件，并且可以在其他B. Cereus群体生物体中找到这些质粒上某些基因的来源吗？复制子之间的基因交换在多大程度上发生了什么机制？ 哪些PXO基因在炭疽芽孢杆菌中是独一无二的？质粒将从基因组DNA的脉冲场凝胶中分离出来，并通过全基因组shot弹枪进行测序，以产​​生完成的高质量序列。 该项目将大大增加有关脑膜外元素如何影响这些重要微生物的关键表型的知识。 这是通过国家科学基金会和农业部之间的合作计划资助的基因组测序奖。