The neural basis of operant conditioning in Aplysia

海兔操作性条件反射的神经基础

• 批准号: 16052427
• 负责人: Professor Dr. Björn Brembs
• 金额: --
• 依托单位: Institut für Zoologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2005
• 资助国家: 德国
• 起止时间: 2004-12-31 至 2009-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/16052427?language=en
• 关键词: neural; basis; operant; conditioning; Aplysia

Learning about the consequences of one s own behavior (operant conditioning) and learning about relations in the environment (classical conditioning) constitute the two main forms of predictive, nondeclarative (procedural) learning. These forms of learning are so fundamental and ubiquitous that even invertebrates such as Aplysia possess the capacity to learn operantly and classically. The neural basis of learning has been and remains of considerable interest to the scientific community and the general public. Nevertheless, research in this area has almost exclusively utilized classical conditioning paradigms as model systems within which to address this issue. Despite providing a greater potential for understanding human capacities such as decision-making, volition, planning and other executive functions (as well as a larger medical relevance), mostly technical and conceptual difficulties have prevented the study of operant conditioning to reach the same level of depth as that of classical conditioning. With recent new discoveries and the development of new molecular biological tools, the simple model system Aplysia now provides the opportunity to close this gap. In this proposal we will use representational difference analysis and single cell rt-PCR to identify the genetic basis of operant and classical conditioning of Aplysia feeding behavior. Using this information, we will identify candidate cells for physiological examination.

了解一个自身行为（操作条件）和学习环境中的关系（经典条件）的后果构成了预测性，非定性（程序）学习的两种主要形式。这些形式的学习形式是如此的基本和无处不在，以至于即使无脊椎动物（例如Aplysia）也具有运作和经典学习的能力。学习的神经基础一直对科学界和公众充满兴趣。然而，该领域的研究几乎完全利用经典条件范式作为模型系统来解决此问题。尽管提供了更大的潜力，可以理解人类能力，例如决策，意志，计划和其他执行功能（以及更大的医学相关性），但大多数情况下的技术和概念困难都阻止了对操作条件的研究，从而达到与经典条件相同的深度。随着最新的新发现和新分子生物学工具的开发，简单的模型系统Aplysia现在提供了缩小这一差距的机会。在此提案中，我们将使用代表性差异分析和单细胞RT-PCR来确定操作和经典调节行为的遗传基础。使用这些信息，我们将确定候选细胞进行生理检查。