Biocatalyst Engineering for Maximum Activity in Nonaqueous Media

在非水介质中实现最大活性的生物催化剂工程

• 批准号: 0228145
• 负责人: Douglas Clark
• 金额: $ 37.06万
• 依托单位: University of California-Berkeley
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-01 至 2007-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0228145&HistoricalAwards=false
• 关键词: Biocatalyst; Engineering; Maximum; Activity; Nonaqueous

The overall goals of this research are to achieve rate enhancements of several thousand fold, and to elucidate the underlying mechanism(s) for the activation process. This research will therefore provide new insights into the factors that govern enzyme activity in organic solvents, and will enable enzymes to play an expanded role in industrial processes, including chemicals and pharmaceuticals synthesis, polymer synthesis, drug discovery, and waste treatment, among other emerging applications of biocatalysis. Moreover, the same techniques that lead to improved enzyme function in organic solvents are likely to be of use in the stabilization of dehydrated protein systems, which is a critical issue in biotherapeutic protein formulation and delivery. Thus, this work has broad impact, ranging from biocatalysis to drug discovery to biopharmaceutical formulations.

这项研究的总体目标是实现数千倍的速率提高，并阐明激活过程的基本机制。因此，这项研究将为控制有机溶剂中酶活性的因素提供新的见解，并使酶能够在工业过程中发挥扩展作用，包括化学物质和药物合成，聚合物合成，药物发现和废物处理，以及其他新兴的生物构分析应用。此外，在有机溶剂中导致酶功能改善的相同技术可能在脱水蛋白系统的稳定中使用，这在生物治疗蛋白制剂和递送中是一个关键问题。因此，这项工作具有广泛的影响，从生物催化到药物发现再到生物制药配方。