ME: Collaborative Research: Deployment of Enhanced Stress Responses to Improve Recombinant Expression Systems

ME：协作研究：部署增强的应激反应以改进重组表达系统

• 批准号: 0224733
• 负责人: Douglas Clark
• 金额: $ 36.59万
• 依托单位: University of California-Berkeley
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至 2006-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0224733&HistoricalAwards=false
• 关键词: ME; Collaborative; Research; Deployment; Enhanced

The objective of this project is to improve recombinant expression systems. The major goals are to discover and characterize pathways that facilitate protein folding and subunit assembly in hyperthermophiles. The components of these pathways will be inserted into recombinant host cells to preempt induction of stress responses during recombinant expression, and to enhance the stabilization and solubilization of recombinant protein. Building on the approach and findings of the investigators previous research, the specific objectives are: (1) to determine the functions of interacting proteins encoded by the 23 genes in the heat shock regulon of the hyperthermophile, Pyrococcus furiosus, (2) to identify functional complexes of thermostable heat shock proteins and express them in E. coli, yeast and mammalian cells to enhance the durability of these cells, and (3) to determine the effects of combinations of chaperonins on high level recombinant expression of proteins in intact cells as well as cell-free transcription-translation systems.

该项目的目的是改善重组表达系统。 主要目标是发现和表征促进蛋白质折叠和亚基组装的途径。 这些途径的成分将插入重组宿主细胞中，以促进重组表达过程中应激反应的诱导，并增强重组蛋白的稳定和溶解化。 在研究者先前研究的方法和发现的基础上，具体目标是：（1）确定由23个基因在高疗中的热休克调节中编码的相互作用蛋白质的功能伴侣蛋白组合对完整细胞中蛋白质高水平重组表达以及无细胞转录 - 翻译系统的影响。