Functional Genomics of Host-Virus Interactions

宿主-病毒相互作用的功能基因组学

• 批准号: 0211872
• 负责人: Savithramma Dinesh-Kumar
• 金额: --
• 依托单位: Yale University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0211872&HistoricalAwards=false
• 关键词: Functional; Genomics; Host; Virus; Interactions

The relationship between eukaryotic viruses and their hosts are characteristic of mosthost-pathogen relationships that have co-evolved. The outcomes of virus-host interactions are genetically pre-determined. To produce disease, viruses must enter the host, multiply locally in host tissues, spread from the site of entry, and overcome or evade host immune responses. Plants have evolved various anti-viral defense strategies to clear viral infection. On the other hand, viruses have evolved counter-defense strategies. Therefore, it is important to understand molecular mechanisms of how viruses evade the host's antiviral defenses.A Tobacco Rattle Virus-based virus induced gene silencing (VIGS) system will be used to identify suppressors of viral resistance and susceptibility factors. In addition, a functional proteomics approach will be used to study virus-host interactions. The knowledge gained from these studies will help to combat infectious plant diseases. Protection of crops from disease can significantly improve agricultural production. Application of a plant's own defense mechanism can lead to more effective protection against plant pathogens. Control of pathogen-induced diseases using cellular genes that function in the disease resistance signaling pathway may provide tremendous agricultural benefits and serve the environment by offering an alternative to pesticide use to prevent disease. Tools and information developed in this project will be made available to the scientific community. These resources will assist in the efforts to improve economically important plants like tomato, potato and pepper.DeliverablesAvailable now: pTRV1, pTRV2, pTRV2-GATEWAY, pTRV-NbPDS, pTRV-tomPDS VIGS system. Contact [e-mail] by 9/03: 250-300 sequence verified pTRV2-tomato EST clones 1000-1500 sequence verified pTRV2-Nb-cDNA Agrobacterium TMV-TAP vector

真核病毒与其宿主之间的关系是大多数共同进化的宿主-病原体关系的特征。病毒与宿主相互作用的结果是基因预先决定的。为了产生疾病，病毒必须进入宿主，在宿主组织中局部繁殖，从进入部位传播，并克服或逃避宿主的免疫反应。植物已经进化出各种抗病毒防御策略来清除病毒感染。另一方面，病毒已经进化出反防御策略。因此，了解病毒如何逃避宿主抗病毒防御的分子机制非常重要。基于烟草嘎嘎病毒的病毒诱导基因沉默（VIGS）系统将用于识别病毒耐药性和易感性因素的抑制因子。此外，功能蛋白质组学方法将用于研究病毒与宿主的相互作用。从这些研究中获得的知识将有助于对抗传染性植物病害。保护农作物免受疾病侵害可以显着提高农业产量。应用植物自身的防御机制可以更有效地防御植物病原体。使用在抗病信号通路中起作用的细胞基因来控制病原体引起的疾病可以提供巨大的农业效益，并通过提供使用农药预防疾病的替代方案来服务于环境。该项目开发的工具和信息将提供给科学界。这些资源将有助于改善番茄、马铃薯和辣椒等重要经济作物。现已提供的交付成果：pTRV1、pTRV2、pTRV2-GATEWAY、pTRV-NbPDS、pTRV-tomPDS VIGS 系统。联系方式 [e-mail] 于 9 月 3 日提供： 250-300 个序列验证的 pTRV2-番茄 EST 克隆 1000-1500 个序列验证的 pTRV2-Nb-cDNA 农杆菌 TMV-TAP 载体