Arabidopsis 2010: Using Functional Genomics to Determine How and Why Plants Synthesize Diverse Triterpenoids

拟南芥 2010：利用功能基因组学确定植物如何以及为何合成多种三萜类化合物

• 批准号: 0209769
• 负责人: Seiichi Matsuda
• 金额: $ 169.23万
• 依托单位: William Marsh Rice University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至 2007-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0209769&HistoricalAwards=false
• 关键词: Arabidopsis; 2010; Using; Functional; Genomics

This multidisciplinary collaborative research is aimed at determining how and why plants synthesize diverse triterpenoids. This project will integrate reverse genetics in Arabidopsis, heterologous expression of cDNAs in yeast, spectroscopic and chromatographic structural determination, and gene expression analysis to elucidate the function of Arabidopsis triterpenoid biosynthetic genes and thereby triterpenoid biosynthetic pathways, control mechanisms, and biological function. Three of the characterized triterpenoid biosynthetic enzymes convert a shared substrate to different compounds; other enzymes to be studied may similarly provide structural diversity, metabolize different substrates, or provide means to spatially or temporally control expression and thus product formation. The effects of the mutations on plant growth, development, and triterpenoid composition will facilitate linking each triterpenoid biosynthetic gene with an enzyme of known catalytic activity and a specific biological role. Genes to be studied include apparent oxidosqualene cyclases, farnesyl pyrophosphate synthases, squalene synthases, squalene epoxidases, and cycloeucalenol isomerase. This work will provide a comprehensive accounting of triterpenoid skeletons synthesized by Arabidopsis, establish which compounds are derived from each gene product, and determine the spatial and temporal expression patterns of the various biosynthetic genes. These experiments will not only elucidate the biological importance of triterpenoid diversity, facilitating future modifications for agricultural benefit, but will provide students with broad interdisciplinary training that bridges modern chemistry and biology. The data from these studies will be publicly available on the project web site (http://www.bioc.rice.edu/~bartel/At_triterpenoids.html), and the knockout seeds will be deposited in the ABRC at Ohio State University for distribution to the community.

这项多学科的合作研究旨在确定植物如何以及为什么合成各种三萜类化合物。 该项目将整合拟南芥中的反向遗传学，酵母中CDNA的异源表达，光谱和色谱结构确定以及基因表达分析，以阐明拟南芥三萜类化合物基因的功能以及三萜类化合物的生物合成途径，控制生物合成途径，对照机理和生物学机制和机制。 三种特征的三萜生物合成酶将共享底物转换为不同的化合物。要研究的其他酶可能会类似地提供结构多样性，代谢不同的底物，或提供空间或时间上控制表达并因此形成产物的手段。 突变对植物生长，发育和三萜组成的影响将有助于将每个三萜生物合成基因与已知催化活性和特定生物学作用的酶联系起来。 要研究的基因包括明显的氧化盐环酶，Farneyl Pyrophophate合酶，鳞状合酶，小矛烯环氧酶和环氧甲醇异构酶。 这项工作将为拟南芥合成的三萜骨架提供全面的解释，确定哪些化合物是从每个基因产物中得出的，并确定各种生物合成基因的空间和时间表达模式。 这些实验不仅将阐明三萜多样性的生物学重要性，从而促进未来的农业利益修饰，而且还将为学生提供广泛的跨学科培训，从而弥补现代化学和生物学。 这些研究的数据将在项目网站（http://www.bioc.rice.edu/~bartel/at_triterpenoids.html）上公开获取，淘汰种子将存放在ABRC中，将其存入俄亥俄州立大学的ABRC。