Arabidopsis 2010: Developing Paradigms for Functional Genomics of Protein Kinases and Phosphoproteins Using the CDPK Superfamily

拟南芥 2010：使用 CDPK 超家族开发蛋白激酶和磷蛋白功能基因组学范例

• 批准号: 0114769
• 负责人: Alice Harmon
• 金额: --
• 依托单位: University of Florida
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2007-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0114769&HistoricalAwards=false
• 关键词: Arabidopsis; 2010; Developing; Paradigms; Functional

Abstract Protein phosphorylation is a major mode of regulation of metabolism, gene expression, and cellular architecture in eukaryotic cells, and defining phosphorylation-based regulatory networks is essential for understanding the function of the Arabidopsis genome. The goal of this project is to define phosphorylation networks that are related to the function of calcium-dependent protein kinases (CDPKs) and four closely related families; CDPK-related kinases (CRKs), phosphoenolpyruvate carboxylase kinases (PPCKs), PPCK-related kinases (PEPRKs), and SNF-1 related kinases (SnRKs). These 88 kinases, which represent about 9% of all the protein kinases in the Arabidopsis genome, are involved in all aspects of plant development and physiology and participate in the coupling of cellular responses to environmental and developmental signals. A list of these protein kinases including gene identification numbers is available at http://www.arabidopsis.org, and further information including links to database records is available at http://plantsp.sdsc.edu. This research will investigate the function of 64 members of these families through determination of the subcellular location of each kinase and identification of downstream targets and other proteins with which the kinases associate. This information will give insight into the physiological roles of each kinase by identifying signaling networks in which each participates. This research will also determine the target sequences in substrate proteins that are phosphorylated by each kinase, and these results will contribute to understanding the overlap in kinase function and cross-talk between signaling pathways. The results of this work will be made available on a yearly basis at the two URLs given above.

抽象蛋白质磷酸化是真核细胞中代谢，基因表达和细胞结构的主要方式，定义基于磷酸化的调节网络对于理解拟南芥基因组的功能至关重要。该项目的目的是定义与依赖钙依赖性蛋白激酶（CDPK）和四个密切相关的家族功能相关的磷酸化网络；与CDPK相关的激酶（CRK），磷酸丙酮酸羧化酶激酶（PPCKS），PPCK相关激酶（PEPRKS）和SNF-1相关激酶（SNRKS）。这88种激酶代表拟南芥基因组中所有蛋白质激酶的9％，参与植物发育和生理学的各个方面，并参与了细胞对环境和发育信号的反应的耦合。这些蛋白激酶（包括基因识别数字）的列表可在http://www.arabidopsis.org上找到，并在http://plantsp.sdsc.edu上获得了包括指向数据库记录的链接的更多信息。这项研究将通过确定每种激酶的亚细胞位置，并鉴定与激酶相关的下游靶标和其他蛋白质的鉴定，研究这些家族64个成员的功能。这些信息将通过识别每个参与的信号网络来洞悉每种激酶的生理作用。这项研究还将确定每种激酶磷酸化的底物蛋白中的靶序列，这些结果将有助于理解激酶功能的重叠和信号通路之间的串扰。这项工作的结果将在上面给出的两个URL上每年提供。