BAC Library: Diversity of Teleost Fishes

BAC 图书馆：硬骨鱼类的多样性

• 批准号: 0208343
• 负责人: Thomas Kocher
• 金额: $ 15万
• 依托单位: University of New Hampshire
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2004-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0208343&HistoricalAwards=false
• 关键词: BAC; Library; Diversity; Teleost; Fishes

0208343KocherTeleost fishes are dominant components of aquatic ecosystems around the world, and are of tremendous commercial importance as a major source of animal protein. Teleosts have undergone a spectacular adaptive radiation over the last 200MY. The 25,000 species of teleost represent more than half of all living vertebrate taxa. Fishes are important model systems across the spectrum of biological research, from ecology, evolution and development to biomedicine. The genomic tools developed to sequence the human genome are now finding applications across many taxa. The genomes of three fish species (Danio, Fugu and Tetraodon) are being completely sequenced. Genetic maps have been constructed for a dozen other species, and physical maps are under construction for a few of these. BAC libraries for additional species will further research aimed at discovering the phylogenetic relationships among species, the genetic basis for adaptive differences among taxa, and the patterns and processes that regulate genome evolution. This project aims to further stimulate comparative genomic studies of fishes by constructing and distributing large-insert genomic DNA (BAC) libraries for two species representing major teleost lineages. They are the blind cavefish (Astyanax mexicanus), and the Lake Malawi zebra (Metriaclima zebra). These BAC libraries will permanently archived and distributed through the UNH Hubbard Center for Genome Studies, a new genome center focused on comparative and environmental genomics. Each library will be distributed as clones in 384-well plates and gridded filters suitable for hybridization screening. Extensive quality control measures will ensure that each derived product (replica plate or filter) can be traced back to the original clone. The Hubbard Center is committed to the development of comparative genomic resources for fishes. This proposal is the first step in a program to generate genomic and cDNA libraries, genetic and physical maps, and comparative mapping databases for fishes. The libraries proposed here will serve as a foundation for future research in phylogenetics, genome evolution, development, evolution, environmental biology and aquaculture.__

0208343Kochererost鱼是世界各地水生生态系统的主要成分，作为动物蛋白的主要来源，其商业意义极为重要。 在过去的200my上，硬骨鱼经历了壮观的自适应辐射。 25,000种的遗产量代表了所有活脊椎动物分类单元中的一半以上。鱼类是整个生物学研究范围内的重要模型系统，从生态，进化和发育到生物医学。现在开发的旨在对人类基因组进行测序的基因组工具现在正在发现许多分类单元的应用。 已经完全测序了三种鱼类（Danio，Fugu和Tetraodon）的基因组。已经为其他十几个物种构建了遗传图，其中一些物理图正在建设中。 其他物种的BAC文库将进一步研究，旨在发现物种之间的系统发育关系，分类单元之间适应性差异的遗传基础以及调节基因组进化的模式和过程。该项目旨在通过构建和分布大型插入基因组DNA（BAC）文库来进一步刺激鱼类的比较基因组研究，以代表主要的遗传谱系。它们是盲人洞鱼（Astyanax Mexicanus）和马拉维湖斑马（Metriaclima Zebra）。 这些BAC库将通过Hubbard基因组研究中心永久存档和分布，这是一个针对比较和环境基因组学的新基因组中心。 每个库将在384孔板中以克隆的形式分布，并将网格的过滤器分布在适合杂交筛选的网格板中。广泛的质量控制措施将确保可以将每个派生产品（复制板或过滤器）追溯到原始克隆。哈伯德中心致力于开发鱼类比较基因组资源。 该提案是生成基因组和cDNA文库，遗传和物理图以及鱼类比较映射数据库的程序的第一步。 这里提出的图书馆将成为系统发育，基因组进化，发展，进化，环境生物学和水产养殖的未来研究的基础。