CAREER: Flow Modulation of Receptor-Mediated Polymorphonuclear Leukocyte-Tumor Cell Interactions: A Research and Education Program

职业：受体介导的多形核白细胞-肿瘤细胞相互作用的流量调节：研究和教育项目

• 批准号: 0093524
• 负责人: Konstantinos Konstantopoulos
• 金额: $ 37.5万
• 依托单位: Johns Hopkins University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-01 至 2006-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0093524&HistoricalAwards=false
• 关键词: CAREER; Flow; Modulation; Receptor; Mediated

PI's objectives are to: (1) Investigate the kinetics of PMN-tumor cell aggregate formation as a function of shear rate, and states of PMN/tumor cell activation. These data will be analyzed using a mathematical model based on Smoluchowski's two-body collision theory that will provide numerical estimates of an important cellular property termed adhesion efficiency, which reflects the binding affinity of interacting cells. (2) Elucidate the fundamental molecular and signaling mechanisms of PMN-tumor cell adhesive interactions using function blocking monoclonal antibodies and highly specific enzymes will, (3) Track individual PMNs in shear flow and delineate the dynamics and pattern of their interactions with surface-anchored tumor cells using a perfusion chamber coupled with an epifluorescence videomicroscopy system.For the educational component of this proposal, the PI aims to develop an innovative university-wide curriculum in Cellular Engineering at both the undergraduate and graduate levels. This goal will be met through the development of a cohesive set of lecture courses and the establishment of a capstone laboratory course in which students will obtain valuable hands-on experience, by being exposed to a wide array of experimental tools and techniques used in bioengineering research. To promote exposure of Bioengineering to high-school students, an outreach program will be developed in parallel. The strategy involves the educational enrichment of regional high-school teachers and the development of an internet-based module using animation and audio/video clips on selected topics of Cellular Engineering in an effort to introduce this material as a section in an advanced science course.

PI的目标是：（1）研究PMN肿瘤细胞骨料形成的动力学，这是剪切速率的函数以及PMN/肿瘤细胞激活的状态。这些数据将使用基于Smoluchowski的两体碰撞理论的数学模型来分析，该模型将提供称为粘附效率的重要细胞性质的数值估计，这反映了相互作用细胞的结合亲和力。 （2）使用功能阻断单克隆抗体和高度特定的酶阐明PMN肿瘤细胞粘合剂相互作用的基本分子和信号传导机制，（3）（3）在剪切流中跟踪各个PMN，并使用与表面群体的组合进行灌注的动力学和模式，并将其与Epercomention Commenty Commenty councomention Commoction Commoction Commoction Commoction Commoction Commoction Commoction Commoction conforcomementions缩减。在该提案中，PI旨在在本科和研究生级别开发创新的大学范围内蜂窝工程课程。通过开发一系列有凝聚力的演讲课程以及建立顶峰实验室课程，学生将通过暴露于生物工程研究中使用的广泛的实验工具和技术来实现这一目标。为了促进生物工程对高中生的接触，将同时制定推广计划。该策略涉及区域高中教师的教育丰富，以及使用动画和音频/视频片段开发基于互联网的模块，涉及蜂窝工程的选定主题，以便将此材料作为高级科学课程中的部分引入。