Integrative Research Program in Marine Genomics

海洋基因组学综合研究计划

• 批准号: 0083102
• 负责人: Eric Lacy
• 金额: $ 50万
• 依托单位: Medical University of South Carolina
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0083102&HistoricalAwards=false
• 关键词: Integrative; Research; Program; Marine; Genomics

This project lays out an innovative program to use genomic and biochemical approaches to investigate genes and gene products in commercial shrimp associated with their resistance to environmental stresses and viral infections. Genomics tools such as the functional analysis of expressed proteins (deduced from the comparative analysis of expressed sequence tags or ESTs) will be developed and applied to explore which genes are involved in resistance and how these genes are regulated. The initial work will focus on the host response of commercial shrimp species to two virus diseases (TSV and WSSV); later the methods may be applied to other marine species as well. A multi-institutional network of researchers at the Medical University of South Carolina, the SC Department of Natural Resources, and the National Ocean Services Laboratory will establish the program building on expertise already in place on molecular genetic and protein biochemical approaches to one aspect of shrimp physiology, namely, the host response. The research effort is directed towards "functional genomics," i.e., the detection and study of genes by virtue of their expression. The work presents an integrated approach for understanding the combined and interacting effects of environmental stresses and viral infection in both cultivated and wild shrimp species, focusing on gene expression as studies by cDNA to mRNA ("ESTs"), and by rapid identification of expressed proteins using mass spectrometry-based protein structure analysis. The current application proposes to put infrastructure in place to: (1) focus efforts on a single species which will lay the groundwork for expansion into other marine species; (2) increase competitiveness by hiring two new additional junior faculty to augment and complement existing capability in molecular biology/genomics and bioinformatics; (3) develop a bioinformatics component which will lay the groundwork for further research and graduate training efforts, and (4) form partnerships with private companies to transfer the findings for commercial interests.

该项目提出了一种创新计划，使用基因组和生化方法研究与环境应激和病毒感染的耐药性相关的商业虾中的基因和基因产物。将开发并应用基因组学工具，例如表达蛋白的功能分析（从表达序列标签或EST的比较分析中得出），并应用于探索哪些基因参与抗性以及如何调节这些基因。最初的工作将集中于商业虾物种对两种病毒疾病（TSV和WSSV）的宿主反应；后来，该方法也可以应用于其他海洋物种。南卡罗来纳州医科大学，SC自然资源系和国家海洋服务实验室的研究人员的多机构研究人员将建立有关已经在分子遗传和蛋白质生化方法上的专业知识的计划，以实现虾生理学的一方面，即宿主的反应。研究工作是针对“功能基因组学”的，即通过其表达来检测和研究基因。这项工作提出了一种综合方法，用于理解栽培和野生虾物种中环境应激和病毒感染的联合和相互作用的作用，重点是cDNA至mRNA的研究，并通过基于质谱蛋白质蛋白质的蛋白质结构分析来快速鉴定表达蛋白质（“ ESTS”）。当前的应用建议将基础设施置于以下位置：（1）将精力集中在将扩展到其他海洋物种的基础上的单一物种上； （2）通过雇用两个新的初级教师来增强和补充分子生物学/基因组学和生物信息学的现有能力来提高竞争力； （3）开发一个生物信息学组成部分，将为进一步的研究和研究生培训工作奠定基础，（4）与私人公司建立合作伙伴关系，以转移有关商业利益的调查结果。