Characterization of New Stingray Sperm Motility Proteins

新黄貂鱼精子运动蛋白的表征

• 批准号: 9420328
• 负责人: Eric Lacy
• 金额: $ 13.05万
• 依托单位: Medical University of South Carolina
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-06-01 至 1997-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9420328&HistoricalAwards=false
• 关键词: Characterization; New; Stingray; Sperm; Motility

9420328 Lacy The motility of sperm is an extremely important factor in determining successful fertilization in livestock, game animals, and man. Numerous drugs have been used to increase sperm motility but each has a major drawback which includes damage to genetic material, producing defects in the embryo, and instability of the drugs. Dr. Lacy's laboratory has discovered a reproductive gland (alkaline gland) in a cartilagenous fish (the stingray) which produces a fluid that accelerates sperm motility not only in stingrays but also in other fish (bass), bull, and man. Experiments in Dr. Lacy's laboratory further showed that the substance in this fluid which stimulates sperm motility appears to be protein in nature. Antibodies to one particular protein, the hormone relaxin, showed that it is likely to be present in this fluid and may be the causative molecule for increased sperm motility. The objective of the current grant application is to determine the mode of action and physiological significance of alkaline gland fluid protein with attention paid to relaxin. Studies will focus on three specific aims: 1) to isolate and purify the sperm stimulating protein(s); 2) to isolate and identify the molecules on the sperm surface which specifically bind the alkaline gland fluid protein(s); and 3) to determine if stingray relaxin has other effects such as contraction of female uterine smooth muscle which would also aid fertilization. The significance of this study's results will be to elucidate some of the mechanisms for successful fertilization in these fish. ***

9420328蕾丝精子的运动是确定牲畜，游戏动物和人成功受精的极为重要的因素。 许多药物已被用来增加精子运动性，但每种药物都有一个主要的缺点，其中包括对遗传物质的损害，在胚胎中产生缺陷以及药物的不稳定性。 蕾丝博士的实验室发现了一种软骨鱼（黄貂鱼）中的生殖腺（碱性腺），该腺产生一种液体，该液体不仅在黄貂鱼中，而且在其他鱼类（鲈鱼），公牛和人类中加速了精子运动。 Lacy博士实验室的实验进一步表明，这种刺激精子运动的液体中的物质似乎是蛋白质的。 一种特定蛋白质的抗体，激素松弛素，表明它可能存在于该液体中，并且可能是增加精子运动的病因分子。 当前赠款应用的目的是确定碱性腺体流体蛋白的作用方式和生理意义，并注意放松蛋白。 研究将重点介绍三个特定目的：1）分离和纯化刺激蛋白的精子； 2）分离并鉴定精子表面上的分子，该分子特异性结合了碱性腺体流体蛋白（S）； 3）确定黄貂鱼松弛素是否具有其他作用，例如女性子宫平滑肌的收缩，这也将有助于受精。 这项研究结果的意义将是阐明这些鱼类成功受精的某些机制。 ***