Structure and Function of Reproductive Fluid Proteins

生殖液蛋白的结构和功能

• 批准号: 9816747
• 负责人: Eric Lacy
• 金额: $ 24.3万
• 依托单位: Medical University of South Carolina
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-02-01 至 2003-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9816747&HistoricalAwards=false
• 关键词: Structure; Function; Reproductive; Fluid; Proteins

9816747LacySuccessful fertilization and production of viable offspring in fish is determined by a number of factors. Important is the transfer of large numbers of highly motile, viable sperm to the female fish or to the eggs deposited into the water. The objectives of the present proposal are to investigate the structure and function proteins in fluids of a cartilaginous fish species which increase sperm motility and relax these fish's uterine smooth muscle. Specific goals are to: 1) Characterize the relaxation response of the fish uterine smooth muscle to the newly purified "raylaxin" molecule in male reproductive fluids and to isolate and characterize its receptor in the cell membrane of the uterine cells. 2) Identify and characterize the sperm stimulatory molecules in the fluids secreted over the fish sperm at the time of mating. Analytical chemistry techniques and microscopy will be used to achieve these goals. The results of these studies should have practical application in fisheries' management as well as contribute to the knowledge of basic biological mechanisms of reproduction.

9816747 lacysuccessuccessful Fustrigation和FISH中可行后代的产生由许多因素决定。 重要的是将大量高度运动，可行的精子转移到雌鱼或沉积在水中的卵。 本提案的目的是研究软骨鱼类的流体中的结构和功能蛋白，这些蛋白会增加精子运动并放松这些鱼的子宫平滑肌。 具体目标是：1）表征鱼子宫平滑肌对雄性生殖液中新纯化的“雷拉辛”分子的松弛反应，并在子宫细胞的细胞膜中分离并表征其受体并表征其受体。 2）识别并表征交配时在鱼精子上分泌的液体中的精子刺激分子。 分析化学技术和显微镜将用于实现这些目标。 这些研究的结果应该在渔业管理中具有实际应用，并有助于了解基本生物学机制。