XYZ on a Chip: Engineering of Innovative Molecular Sieves on a Chip by Nanoprint Lithography

芯片上的 XYZ：通过纳米印刷光刻技术在芯片上设计创新分子筛

• 批准号: 9980814
• 负责人: Robert Austin
• 金额: $ 45.99万
• 依托单位: Princeton University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-10-01 至 2003-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9980814&HistoricalAwards=false
• 关键词: XYZ; Chip; Engineering; Innovative; Molecular

9980814AustinThe PI's propose to develop a pioneering technology which exploits specifically a combination of nanotechnology, new physics in fractionation technology and single-molecule molecular biology. They believe that only by breaking away from conventional techniques can the true potential of chip technology be exploited. The two principle investigators for this project are superbly qualified to carry out this work. Professor Stephen Chou (Department of Electrical Engineering) is a world leader in nanofabrication technologies and has pioneered the development of nanoimprint lithography while Professor Robert Austin (Department of Physics) has been a pioneer in the applications of microfabrication in molecular and cellular biology.Most efforts at using chip technology to do sequencing of DNAmolecules have concentrated on the shrinking of capillary electrophoresis channels onto the surface of silicon wafer. The problem with this technology is that without creating explicitly on the chip a change in the basic polymer physics by which the molecules are fractionated the resolving power of the devices is limited by the short length of the channels on the wafer. In other words, these efforts do not take full advantage of the design freedom that lithographic techniques offer to an imaginative designer. A more powerful aspect of chip technology is also the potential to do single molecule genomic analysis in a massively parallel manner using nanoscale engineering and nanoimprint lithography.The proposed work consists of 3 related projects: (1) nanofabrication of obstacle arrays with characteristic spacings of 30-600 manometers to be used for the construction of synthetic gels for fractionation of oligonucleotides; (2) nanofabrication of asymmetrical arrays which use rectified brownian motion for the continuous fractionation of biological molecules; (3) nanofabrication of near-field excitation slits and channels for ultra-high spatial reading of tagged genomic DNA.This proposal offers the next step forward in the engineering of nanostructures on chip for the fractionation of biological molecules and the sequencing of single molecules. The technology described could become the basis for the future evolution of nanobiology and nanoengineering of a biology lab on a chip.***

9980814 Austinthe Pi提议开发一种开拓技术，该技术专门利用了纳米技术，分离技术的新物理学和单分子分子生物学的组合。 他们认为，只有摆脱传统技术，才能利用芯片技术的真正潜力。 该项目的两个主要调查人员有资格进行这项工作。 斯蒂芬·朱（Stephen Chou）教授（电气工程系）是纳米制造技术的世界领导者，并开创了纳米印刷印刷术的发展，而罗伯特·奥斯汀（Robert Austin）教授（物理学）一直是在分子和细胞生物学中的大多数努力来使用Semercort of Semercill of sequilcill of sequilcercill of sequencern inninm inmink ninm inminn inker ninm inker ninm inmink in ninm inmink nink nink nink nink nink nink nink nink nink nink nink，电泳通道到硅晶片的表面。 这项技术的问题在于，如果不明确地在芯片上创建基本聚合物物理学的变化，则分子被分馏的基本聚合物物理学，设备的分辨能力受到晶圆上的通道的短长限制。 换句话说，这些努力并不能充分利用光刻技术为富有想象力的设计师提供的设计自由。 A more powerful aspect of chip technology is also the potential to do single molecule genomic analysis in a massively parallel manner using nanoscale engineering and nanoimprint lithography.The proposed work consists of 3 related projects: (1) nanofabrication of obstacle arrays with characteristic spacings of 30-600 manometers to be used for the construction of synthetic gels for fractionation of oligonucleotides; （2）不对称阵列的纳米化阵列，这些阵列使用整流的布朗运动来连续分馏生物分子； （3）对标记的基因组DNA进行超高空间读数的近场激发缝隙和通道的纳米化。该提案为芯片上的纳米结构提供了下一步，以分馏生物分子的分馏和单分子的测序。 所描述的技术可能成为纳米生物学和纳米工程生物学实验室的未来进化的基础。*** ***