Microlithographic Manipulation of Macromolecules

高分子的微光刻操作

• 批准号: 9202170
• 负责人: Robert Austin
• 金额: $ 42.5万
• 依托单位: Princeton University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-01 至 1997-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9202170&HistoricalAwards=false
• 关键词: Microlithographic; Manipulation; Macromolecules

Microlithographic quasi-two-dimensional complex surfaces will be constructed through which DNA will be electrophoresed. The motions of large single molecules through these obstacle arrays will be observed using real-time fluorescence imaging techniques. %%% These devices should allow the fractionation of DNA molecules at the chromosome length scale in a highly reproducible environment. The ability to bind and move very long DNA molecules through synthetic microlithographic surfaces may be used to align chromosomal length DNA molecules on complex designed surfaces, to analyze the DNA sequence using scanning techniques and to probe DNA structure using optical sensors. The overall aim of the project is single molecule processing.

将构建微柱准二维复合体表面，通过该表面将通过该表面进行电泳。 将使用实时荧光成像技术观察到大型单分子在这些障碍阵列中的运动。 这些设备应允许在高度可重复的环境中以染色体长度尺度上的DNA分子分馏。 通过合成微观表面结合和移动非常长的DNA分子的能力可用于将染色体长度的DNA分子对齐在复杂设计的表面上，以使用扫描技术和使用光学传感器探测DNA序列。 该项目的总体目的是单分子加工。