Protein Transport and Chromatography with Gel-Composite Ion-Exchangers

使用凝胶复合离子交换剂进行蛋白质运输和色谱分析

• 批准号: 9709670
• 负责人: Giorgio Carta
• 金额: $ 16万
• 依托单位: University of Virginia Main Campus
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-10-01 至 2001-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9709670&HistoricalAwards=false
• 关键词: Protein; Transport; Chromatography; Gel; Composite

Abstract Proposal No: 9709670 Proposal Type: Investigator Initiated Principal Investigator: Giorgio Carta Affiliation: University of Virginia This grant is awarded through the Separations and Purification Program sub-element of the Interfacial, Transport and Separations Program of the Chemical and Transport Systems Division. The principal investigator is Dr. Giorgio Carta of the University of Virginia. The research is concerned with the characterization of diffusional transport of proteins and other macromolecules in gel-composite ion-exchangers and with the use of these materials in protein chromatography. The materials are obtained by synthesizing functionalized polymer gels within the pores of a rigid support matrix such that an essentially complete filling of the pores is achieved. The gel is thus stable to mechanical forces and to changes in solution conditions, allowing chromatographic operations at elevated flow rates of the mobile phase. This research addresses the development of new technologies for process scale protein chromatography. The fundamental understanding of protein transport in gels will aid development of new protein separation technologies, especially those oriented to high volume rapid separations.

摘要建议编号：9709670建议类型：研究人员发起的首席研究者：Giorgio Carta隶属关系：弗吉尼亚大学这笔赠款是通过分离和净化计划的界面，交通和分离计划的分离和净化计划子元素授予的。主要研究人员是弗吉尼亚大学的乔治·卡塔（Giorgio Carta）博士。该研究与凝胶复合离子交换器中蛋白质和其他大分子的扩散转运以及在蛋白质色谱中使用这些材料的表征有关。这些材料是通过合成刚性支撑矩阵孔中的功能化聚合物凝胶获得的，从而实现了孔基本上完整的填充。因此，凝胶对机械力和溶液条件的变化稳定，从而在流动相升高的流速下进行色谱操作。 这项研究介绍了用于过程规模蛋白质色谱法新技术的开发。凝胶中蛋白质转运的基本了解将有助于开发新的蛋白质分离技术，尤其是针对大量快速分离的蛋白质分离技术。