Protein Transport and Chromatography with Gel-Composite Ion-Exchangers

使用凝胶复合离子交换剂进行蛋白质运输和色谱分析

• 批准号: 9709670
• 负责人: Giorgio Carta
• 金额: $ 16万
• 依托单位: University of Virginia Main Campus
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-10-01 至 2001-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9709670&HistoricalAwards=false
• 关键词: Protein; Transport; Chromatography; Gel; Composite

Abstract Proposal No: 9709670 Proposal Type: Investigator Initiated Principal Investigator: Giorgio Carta Affiliation: University of Virginia This grant is awarded through the Separations and Purification Program sub-element of the Interfacial, Transport and Separations Program of the Chemical and Transport Systems Division. The principal investigator is Dr. Giorgio Carta of the University of Virginia. The research is concerned with the characterization of diffusional transport of proteins and other macromolecules in gel-composite ion-exchangers and with the use of these materials in protein chromatography. The materials are obtained by synthesizing functionalized polymer gels within the pores of a rigid support matrix such that an essentially complete filling of the pores is achieved. The gel is thus stable to mechanical forces and to changes in solution conditions, allowing chromatographic operations at elevated flow rates of the mobile phase. This research addresses the development of new technologies for process scale protein chromatography. The fundamental understanding of protein transport in gels will aid development of new protein separation technologies, especially those oriented to high volume rapid separations.

摘要 提案编号：9709670 提案类型：研究者发起 首席研究员：Giorgio Carta 隶属关系：弗吉尼亚大学 这项资助是通过化学和传输系统部门界面、传输和分离计划的分离和纯化计划子要素授予的。主要研究者是弗吉尼亚大学的 Giorgio Carta 博士。该研究涉及凝胶复合离子交换剂中蛋白质和其他大分子的扩散运输特性以及这些材料在蛋白质色谱中的应用。该材料是通过在刚性支撑基质的孔内合成官能化聚合物凝胶而获得的，从而实现孔的基本完全填充。因此，凝胶对机械力和溶液条件的变化是稳定的，允许在流动相的较高流速下进行色谱操作。 这项研究致力于开发过程规模蛋白质色谱新技术。对凝胶中蛋白质转运的基本了解将有助于开发新的蛋白质分离技术，特别是那些面向大容量快速分离的技术。