Protein Transport in Charged Gels for Chromatography and Membrane Applications

用于色谱和膜应用的带电凝胶中的蛋白质运输

• 批准号: 0414143
• 负责人: Giorgio Carta
• 金额: $ 27.7万
• 依托单位: University of Virginia Main Campus
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-15 至 2008-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0414143&HistoricalAwards=false
• 关键词: Protein; Transport; Charged; Gels; Chromatography

AbstractProposal Title: Protein Transport in Charged Gels for Chromatography and Membrane ApplicationsProposal Number: CTS-0414143Principal Investigator: Georgio CartaInstitution: University of VirginiaThe objective of this project is to develop a fundamental understanding of protein partitioning and transport in charged hydrogels. Understanding the transport properties of charged macromolecules in such materials is a key to their development and optimum design. New insights have recently been obtained through microscopic studies, which can provide the basis to develop a mechanistic understanding of transport. This project will expand current efforts in this area with the ultimate goal of developing new adsorbents and membranes for protein separations as well as models to predict their process performance. Protein partitioning and transport in charged dextran gels and agarose-dextran composites will be studied on a microscopic scale focusing on the effects of gel properties and protein molecular weight and charge on equilibrium uptake and mass transfer kinetics. Kinetics of protein ion-exchange in charged poly(acrylamide) gels and charged agarose-dextran composites will be studied using radiotracer techniques to elucidate protein transport under both gradient and tracer diffusion conditions. Modeling single and multicomponent protein transport in ionic gels and application of these models to predict the behavior of column chromatography under processing conditions will be performed. This research will provide a higher level of understanding of transport phenomena in these complex media as well as models to aid the design and optimization of chromatographic processes. The research will have multiple broader impacts. It will guide the development of optimized stationary phases by media manufacturers. It will also provide a basis for the formulation of rate models to predict and optimize chromatographic separations permitting pharmaceutical companies to improve the design and efficiency of bioprocesses. Since separation and purification are often the costliest steps in bioprocessing, there is a critical need for separation scientists and engineers who are trained to develop and implement novel separation media integrating a broad spectrum of experimental and modeling approaches to improve the effectiveness of industrial bioseparations.

摘要提案标题：用于色谱和膜应用的带电凝胶中的蛋白质运输提案编号：CTS-0414143主要研究员：Georgio Carta机构：弗吉尼亚大学该项目的目标是对带电水凝胶中蛋白质的分配和运输有一个基本的了解。 了解此类材料中带电大分子的传输特性是其开发和优化设计的关键。 最近通过微观研究获得了新的见解，这可以为发展对运输的机制理解提供基础。 该项目将扩大该领域目前的努力，最终目标是开发用于蛋白质分离的新型吸附剂和膜以及预测其工艺性能的模型。 将在微观尺度上研究带电葡聚糖凝胶和琼脂糖-葡聚糖复合材料中的蛋白质分配和运输，重点研究凝胶特性、蛋白质分子量和电荷对平衡吸收和传质动力学的影响。 将使用放射性示踪剂技术研究带电聚（丙烯酰胺）凝胶和带电琼脂糖-葡聚糖复合材料中蛋白质离子交换的动力学，以阐明梯度和示踪剂扩散条件下的蛋白质转运。 将对离子凝胶中的单组分和多组分蛋白质运输进行建模，并应用这些模型来预测处理条件下柱色谱的行为。 这项研究将提供对这些复杂介质中传输现象以及模型的更高水平的理解，以帮助色谱过程的设计和优化。 该研究将产生多种更广泛的影响。 它将指导介质制造商开发优化的固定相。 它还将为速率模型的制定提供基础，以预测和优化色谱分离，从而使制药公司能够改进生物工艺的设计和效率。由于分离和纯化通常是生物加工中成本最高的步骤，因此迫切需要经过培训的分离科学家和工程师，以开发和实施集成广泛实验和建模方法的新型分离介质，以提高工业生物分离的有效性。