Replacement of a 500 MHz NMR Console at Florida State University

更换佛罗里达州立大学的 500 MHz NMR 控制台

• 批准号: 9604787
• 负责人: Timothy Logan
• 金额: $ 24.03万
• 依托单位: Florida State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-02-01 至 1999-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9604787&HistoricalAwards=false
• 关键词: Replacement; 500; MHz; NMR; Console

Funds are requested to replace an existing Varian VXR 500-S 500 MHz NMR spectrometer console, which is part of the Chemistry Department NMR Facility at Florida State University. Our objective is to replace the existing console with one having three independent radio frequency (rf) channels, waveform generators for each rf channel, and a three-axis pulsed-field gradient accessory. To take advantage of the additional hardware capabilities, we will also purchase a total of three new double- and triple-resonance probes and an off-line Silicon Graphics Indy workstation out-fitted with an external hard-drive and a CD recorder. The 11.7 T Oxford super-conducting magnet, which has very good drift and lineshape specifications, will not be replaced. To optimize the lineshape of the water resonance in biological samples, a new room-temperature shim set will be purchased. This course of action will substantially enhance the overall capabilities of the instrument in a cost-effective manner, while retaining the desirable functional components of the existing system. The replacement spectrometer console will be used for solution studies of ( 1 ) glycoprotein structural biology, (2) protein folding, (3) protein-solvent interactions, (4) RNA structure, (5) structures of RNAprotein complexes, and (6) larger organic molecules.

需要资金来更换现有的瓦里安 VXR 500-S 500 MHz NMR 波谱仪控制台，该控制台是佛罗里达州立大学化学系 NMR 设施的一部分。 我们的目标是用具有三个独立射频 (rf) 通道、每个射频通道的波形发生器以及三轴脉冲场梯度附件的控制台来替换现有控制台。为了利用额外的硬件功能，我们还将购买总共三个新的双谐振和三谐振探头以及一个配备外部硬盘驱动器和 CD 刻录机的离线 Silicon Graphics Indy 工作站。 11.7T牛津超导磁体，具有非常好的漂移和线型规格，不会被更换。为了优化生物样品中水共振的线形，将购买一套新的室温垫片组。这一行动方案将以经济高效的方式显着增强仪器的整体功能，同时保留现有系统所需的功能组件。 替换光谱仪控制台将用于 (1) 糖蛋白结构生物学、(2) 蛋白质折叠、(3) 蛋白质-溶剂相互作用、(4) RNA 结构、(5) RNA 蛋白质复合物结构和 (6) 的溶液研究较大的有机分子。