Methodologies for Analysis of Nucleoprotein Complexes

核蛋白复合物的分析方法

• 批准号: 9509132
• 负责人: Michelle Hanna
• 金额: $ 5万
• 依托单位: University of Oklahoma Norman Campus
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-07-15 至 1996-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9509132&HistoricalAwards=false
• 关键词: Methodologies; Analysis; Nucleoprotein; Complexes

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; R O O O T E N T R Y F（C O M P O B J B J B J B J B J B J B J B J B J B J B J B J B J B J B J B J M E C U C U M E N T O B J E C T P P O O O L 6X 6X 6X 6X +！ e = e * * * n n n n n n n n n n 1 o t; N N N N N N N N N N 4 9509132 HANNA这个项目使用生物物理技术来分析在转录水平上调节基因表达的分子机制，而转录水平是由RNA聚合酶催化的。 这项工作主要集中在大肠杆菌上，这是一个理想的模型系统，可以使用纯化的组件进行机械研究。 该实验室已经开发了几种用光叠链接，荧光或其他标签标记的新型核糖核苷酸类似物。 这些将用于分析大肠杆菌转录复合物中蛋白质核酸的相互作用，使用电子显微镜和荧光极化技术%% %% %% %% %% %% %% %% %% %% %% %%相互作用在许多细胞功能中很重要，包括基因表达的调节。 该项目使用生物物理技术来研究一种蛋白质的酶RNA聚合酶如何附着在细菌的DNA上，并将DNA中的信息转录为RNA，然后成为蛋白质合成的蓝图。 ***哦 +'0 s u m a r y i n f o r m a t i o n（$ h l d h r：\ wwuser \ template \ template \ normal.dot 9509132 Marcia Steinberg Marcia Steinberg Marcia Steinberg @ 2 @ 2 @ 2 @ 2 @ 2 @ 2 @ microsoft Word 6.0 1; *＆ *！ 呢 呢 呢 呢 k @ normal a b c“ a @”默认段落字体 * * * * 4 Marcia Steinberg \\ Clm11 \ McBpub \ McBpub \ Biochem \ biochem \ hanna.abs @hp Deskjet 500 lpt1：hpdskjet hpdskjet hp deskjet hp deskjet 500 d l f f f，，，！ /' ^} w;/'} D G 2 HP Deskjet 500 d L F，，！ /' ^} w;/'} D G 2％％％$ 1 $ 1倍新罗马符号和Arial“ H V \ V \ $＃; 9509132 Marcia Steinberg Marcia Marcia Steinberg;