Post-transcriptional Control of Immunoglobulin Expression

免疫球蛋白表达的转录后控制

• 批准号: 9507513
• 负责人: Martha Peterson
• 金额: $ 30万
• 依托单位: University of Kentucky Research Foundation
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-08-15 至 1999-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9507513&HistoricalAwards=false
• 关键词: Post; transcriptional; Control; Immunoglobulin; Expression

9507513 Peterson Regulated transcriptional termination and RNA processing of the immunoglobulin (Ig) heavy chain ( gene will be examined during B cell development. The long-term goal of this research is to better understand the general mechanisms governing these events as well as to determine the basis for the developmentally regulated changes that occur during B cell maturation. The regulated alternative processing of a ( precursor RNA to form (s and (m mRNA does not require any Ig gene-specific sequences. Instead, the important feature of this gene is that it contains signals for competing splice and cleavage-polyadenylation reactions. Thus, the amount or activity of a general factor(s) involved in these two processes must be altered during B cell maturation. It will be determined whether splicing is regulated during B cell maturation by analyzing alternative splice choices made in the absence of a poly(A) site in B cells as compared to plasma cells. An in vitro splicing/polyadenylation system that will mimic in vivo ( gene characteristics will be examined. Successfully establishing an in vitro system to study ( regulation would provide the basis for many future experiments. The expression of the known general RNA processing regulators in B cells and plasma cells will be examined to identify factors that potentially mediate the regulation; any whose expression correlates with ( processing changes will be studied further by over-expression in B cells and/or plasma cells. These experiments, taken together, will provide a more complete picture of ( processing regulation and should begin to identify the trans-acting RNA processing components that are altered during B cell maturation. To investigate transcriptional termination and its regulation, the transcription of intact and modified ( genes, stably introduced into plasma cell and B cell lines, will be analyzed by nuclear run-on experiments to identify their termination regions. Gene modifications will be designed to determine the contributio n that the two ( poly(A) sites, the poly(A) site strength, and the downstream sequences within the termination region make to the overall termination process. This study, by comparing normal and modified genes in a single cell type will provide information about the general process of termination and, by comparing the modified genes in both B cells and plasma cells, will examine the basis for regulatory changes in this process. %%% Regulated transcriptional termination and RNA processing of the immunoglobulin (Ig) heavy chain ( gene will be examined during B cell development. The long-term goal of this research is to better understand the general mechanisms governing these events as well as to determine the basis for the developmentally regulated changes that occur during B cell maturation. ***

9507513彼得森调节了免疫球蛋白（IG）重链的转录终止和RNA处理（将在B细胞开发过程中检查基因。这项研究的长期目标是更好地了解这些事件的一般机制，以确定在B细胞成熟过程中对B细胞成熟过程中发生的开发的基础（MRNA）（MRNA）（MRNA）（MR）（MR）（MR）（MR）（MR）（MR）的基础。任何Ig基因特异性序列。与浆细胞相比，可以模仿体内的体外剪接/聚腺苷酸化系统（基因特征将被检查。 Successfully establishing an in vitro system to study ( regulation would provide the basis for many future experiments. The expression of the known general RNA processing regulators in B cells and plasma cells will be examined to identify factors that potentially mediate the regulation; any whose expression correlates with ( processing changes will be studied further by over-expression in B cells and/or plasma cells. These experiments, taken together, will provide a more complete picture of ( processing regulation and should begin to确定在B细胞成熟过程中改变的反式RNA加工成分，以研究转录终止及其调节，完整和修饰的转录（稳定引入到血浆细胞和B细胞系中，将通过核跑步的实验来分析，以识别其终止定位。终止区域内的下游序列使得整体终止过程。这项研究通过比较单个细胞类型中的正常基因和修饰的基因将提供有关终止的一般过程的信息，并通过比较B细胞和浆细胞中的修饰基因，将研究此过程中调节性变化的基础。 %%％调节的转录终止和免疫球蛋白（IG）重链的RNA处理（将在B细胞开发过程中检查基因。这项研究的长期目标是更好地了解管理这些事件的一般机制，并确定B细胞成熟过程中发生的开发变化的基础。*** *** *** *** *** *** *** ***。