PECASE: Dielectric Phenotyping of Bacteria for Energy and Medicine

PECASE：用于能源和医学的细菌介电表型分析

• 批准号: 1150615
• 负责人: Cullen Buie
• 金额: $ 40万
• 依托单位: Massachusetts Institute of Technology
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-06-01 至 2017-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1150615&HistoricalAwards=false
• 关键词: PECASE; Dielectric; Phenotyping; Bacteria; Energy

Abstract1150615-Buie, Cullen R.The objective of the proposed research is to illuminate the coupling between cell envelope phenotype and dielectric properties. This will be achieved by the investigation of pathogenic bacteria and electrochemically active bacteria. A three-dimensional insulator based dielectrophoresis (3DiDEP) device will be used. The novel 3DiDEP platform enables non-invasive sorting, trapping, and characterization of living cells based upon the cell envelope phenotype. The hypothesis of the work is that 3DiDEP can be used to characterize the functions expressed on the bacteria cell envelope. 3DiDEP enables low voltage, high sensitivity separation and analysis of cells. Also, the proposed 3DiDEP employs electrodes outside of the microchannel, the technique operates at low frequency or DC electric fields. The proposed 3DiDEP is a powerful technique for high throughput phenotyping in microbiology. Phenotype based selection using 3DiDEP represents one of the first applications of microfluidics in the growing field of phenomics. The proposed project may also help to address widespread concerns in diverse fields ranging from renewable energy to healthcare. The PI also proposes to develop relationships with target universitis to increase the representation of underrepresented minorities in MIT graduate programs. The PI has outlined a unique approach to train a new generation of underrepresented minority scientists who may return to their respective undergraduate institutions as faculty, thereby attacking the diversity issue on both the student and faculty levels.

摘要1150615-Buie，Cullen R.本研究的目的是阐明细胞包膜表型与介电特性之间的耦合。 这将通过对病原菌和电化学活性细菌的研究来实现。 将使用基于三维绝缘体的介电电泳（3DiDEP）装置。 新颖的 3DiDEP 平台能够根据细胞包膜表型对活细胞进行非侵入性分选、捕获和表征。 这项工作的假设是 3DiDEP 可用于表征细菌细胞包膜上表达的功能。 3DiDEP 可实现低电压、高灵敏度的细胞分离和分析。 此外，所提出的 3DiDEP 采用微通道外部的电极，该技术在低频或直流电场下运行。 所提出的 3DiDEP 是一种强大的微生物学高通量表型分析技术。 使用 3DiDEP 进行基于表型的选择代表了微流体在不断发展的表型组学领域的首批应用之一。 拟议的项目还可能有助于解决从可再生能源到医疗保健等各个领域的广泛关注。 PI 还建议与目标大学建立关系，以增加麻省理工学院研究生课程中代表性不足的少数群体的代表性。 PI 概述了一种独特的方法来培训新一代代表性不足的少数族裔科学家，这些科学家可能会返回各自的本科院校担任教职人员，从而解决学生和教职人员层面的多样性问题。