Development and Application of Solid Phase Oligonucleotide Synthesis Supports

固相寡核苷酸合成载体的开发及应用

• 批准号: 9424040
• 负责人: Marc Greenberg
• 金额: $ 25.6万
• 依托单位: Colorado State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-06-01 至 1999-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9424040&HistoricalAwards=false
• 关键词: Development; Application; Solid; Phase; Oligonucleotide

With this award, the Synthetic Organic Program and the Office of multidisciplinary Activities are supporting the research of Dr. Marc M. Greenberg of the Department of Chemistry at Colorado State University. Dr. Greenberg will focus his work on developing solid phase oligonucleotide synthesis supports. The resulting protected oligonucleotides have important biological properties and the work has important biotechnical implications. The focus of this research is the development of orthogonal linkers for solid phase oligonucleotide synthesis that utilize known photochemical and organometallic reactions, and conjugation of the biopolymers released from these novel supports. Conjugation methodology will focus on the formation of bonds between oligonucleotides and peptides as well as between two oligonucleotides. The methodology will be particularly useful for the synthesis of RNA molecules.

通过此奖项，合成有机计划和多学科活动办公室正在支持科罗拉多州立大学化学系的Marc M. Greenberg博士的研究。 格林伯格博士将重点放在开发固相寡核苷酸合成支持上。 由此产生的保护寡核苷酸具有重要的生物学特性，并且该工作具有重要的生物技术意义。 这项研究的重点是利用已知的光化学和有机金属反应的固相寡核苷酸合成的正交连接器的发展，以及从这些新型支持中释放的生物聚合物的结合。 共轭方法将集中于寡核苷酸和肽之间以及两个寡核苷酸之间的键形成。 该方法将对RNA分子的合成特别有用。