Development and Application of Oligonucleotide Synthesis Methods

寡核苷酸合成方法的开发及应用

• 批准号: 0245625
• 负责人: Marc Greenberg
• 金额: $ 1.37万
• 依托单位: Johns Hopkins University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0245625&HistoricalAwards=false
• 关键词: Development; Application; Oligonucleotide; Synthesis; Methods

The proposed research involves the synthesis of novel `orthogonal` solid phase supports. These will be used for the preparation of protected oligonucleotides, and oligonucleotide conjugates with functional groups or peptides attached at defined positions. Related oligoribonucleotides will also be prepared. The research will extend the types of reactions that can be used to cleave the oligonucleotides from the solid support to include reductive and enzymatic reactions, and extend the scope of chemistry available for conjugating protected oligonucleotides. With this renewal award, the Organic and Macromolecular Chemistry Program supports the research of Dr. Marc M. Greenberg of the Department of Chemistry at Colorado State University. The research focuses on developing solid phase supports from which protected fragments of DNA and RNA can be cleaved under mild conditions, and on expanding the repertoire of reactions available for extending the number of nucleotides linked to form the oligomers. The work will provide useful approaches to others attempting to synthesize oligonucleotides, and will have practical biomedical applications. Graduate students and post-doctoral fellows will receive training in the synthesis of small molecules and biopolymers, and get the kind of training needed in the growing biotechnology area.

拟议的研究涉及新型正交固相支持的合成。这些将用于制备受保护的寡核苷酸，以及与定义位置附着的官能团或肽的寡核苷酸结合物。 还将准备相关的寡核苷酸。 这项研究将扩展可用于将寡核苷酸从固体载体切割的反应类型，以包括还原性和酶促反应，并扩展可用于共轭保护的寡核苷酸的化学范围。 通过这一续签奖，有机和大分子化学计划支持科罗拉多州立大学化学系的Marc M. Greenberg博士的研究。 该研究的重点是开发固相支撑，在轻度条件下可以从中裂解受保护的DNA和RNA的片段，并扩大可用于扩大与形成低聚物链接的核苷酸数量的反应的曲目。 这项工作将为尝试合成寡核苷酸的其他人提供有用的方法，并具有实用的生物医学应用。 研究生和博士后研究员将接受有关小分子和生物聚合物合成的培训，并获得成长中生物技术领域所需的培训。