Acquisition of a University Confocal Laser Scanning Microscope System

购置大学共焦激光扫描显微镜系统

• 批准号: 9419609
• 负责人: Robert Jenkins
• 金额: $ 11.9万
• 依托单位: University of Wyoming
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-06-01 至 1997-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9419609&HistoricalAwards=false
• 关键词: Acquisition; University; Confocal; Laser; Scanning

This proposal requests funds to assist in the purchase of a Confocal Laser Scanning Microscope System (CLSM). The enormous and rapidly expanding battery of antibodies and of other fluorescent probes has opened the way to a greatly expanded use of fluorescent microscopy. Unfortunately, the thickness and light scattering occurring in many biological specimens seriously compromises the sensitivity, clarity and resolution of even the best epi-fluorescent images. CLSM overcomes these limitations and produces images of great clarity. This instrumentation will serve the needs of biological scientists at the University of Wyoming (UW) whose work focuses on cell architecture and cellular dynamics, organelle structures and their spatial and temporal relationships and the localizations/functions of cell and tissue antigens. The proposal is submitted by a major user group representing four UW departments: Botany, Molecular Biology, Psychology, and Zoology and Physiology. The system will be available to all UW researchers who have legitimate needs and will operate as a bona fide University instrumentation center. The acquisition of this state-of-the-art instrumentation and the establishment of a CLSM center will offer investigators the opportunity to remain competitive in cell/molecular biology and neuroscience, foster research collaborations and provide training for both undergraduate and graduate students. The CLSM system will initially be used in support of the following projects: Chromosomal Studies in Bromeliaceae (G. Brown, Botany), The Role of Bombesin-like Peptides in Neural Physiology (F. Flynn, Psychology), Applications of Confocal Laser Microscopy to Research on Nuclear Phenomena in Ciliate Protozoa (R. Jenkins, Zoology), Probing Plant-Fungal Interactions: The Form and Physiology of Ectomycorrhizal Fungal Linkages with Plant Roots (S. Miller, Botany), Effects of Heat on Development: Use of the Confocal Microscope to Follow Assembly of Actin Fibers and Localizati on of Integrins Following Heat Shock (N. Petersen, Molecular Biology), Applications of Confocal Microscopy to Research in Behavioral Neuroendocrinology (J. Rose, Psychology), Confocal Microscopy in Studies of DNA Escape from Mitochondria to the Nucleus (P. Thorsness, Molecular Biology), Laser Confocal Microscopy in Studies of Leaf Structure Function: Do Epidermal Lens Cells Play a Role in Photosynthesis? (T. Vogelmann, Botany). In addition, the CLSM system will be used as a teaching tool within laboratory based courses within the four departments as well as for closely supervised graduate and undergraduate research. The total project cost is $253,710 of which the University of Wyoming has pledged a 49% match. NSF FORM 1358 (1/94) 3

该提案要求资金协助购买共聚焦激光扫描显微镜系统（CLSM）。大量抗体和其他荧光探针的巨大和快速扩展的电池为大大扩展的荧光显微镜开辟了道路。 不幸的是，许多生物标本中发生的厚度和光散射严重损害了最佳的荧光图像的敏感性，清晰度和分辨率。 CLSM克服了这些局限性，并产生了明确的图像。该仪器将满足怀俄明大学（UW）生物学科学家的需求，其作品着重于细胞结构和细胞动力学，细胞器结构及其空间和时间关系以及细胞和组织抗原的局部化/功能。该提案由代表四个UW部门的主要用户组提交：植物学，分子生物学，心理学以及动物学和生理学。该系统将适用于所有有合法需求并将作为真正的大学仪器中心运行的UW研究人员。收购这种最先进的仪器以及建立CLSM中心将为调查人员提供在细胞/分子生物学和神经科学，促进研究合作中保持竞争力的机会，并为本科生和研究生提供培训。 CLSM系统最初将用于支持以下项目：溴细菌科（G. Brown，植物学）中的染色体研究，bombesin样肽在神经生理学中的作用（F. Flynn，心理学，心理学），同类激光显微镜在探测器中的核电eTogoza（R. jenkal）的核现象，j r. jenkal，j. jenkal，j. jenkal，j. jenkal，k. and Physiology of Ectomycorrhizal Fungal Linkages with Plant Roots (S. Miller, Botany), Effects of Heat on Development: Use of the Confocal Microscope to Follow Assembly of Actin Fibers and Localizati on of Integrins Following Heat Shock (N. Petersen, Molecular Biology), Applications of Confocal Microscopy to Research in Behavioral Neuroendocrinology (J. Rose, Psychology), Confocal Microscopy在DNA从线粒体逃到细胞核的研究（P. thorsness，Molecular Biology），激光共聚焦显微镜在叶片结构功能的研究中：表皮透镜细胞在光合作用中是否起作用？ （T. Vogelmann，植物学）。 此外，CLSM系统将在四个部门内的实验室课程以及密切监督的研究生和本科研究中用作教学工具。项目总成本为253,710美元，其中怀俄明大学承诺与49％的比赛承诺。 NSF表格1358（1/94）3