Dissertation Research: Molecular Systematics of Artemisia Sect. Tridentatae

论文研究：蒿属植物分子系统学。

• 批准号: 9311086
• 负责人: Linda Watson
• 金额: $ 0.95万
• 依托单位: University of Oklahoma Norman Campus
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-08-01 至 1997-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9311086&HistoricalAwards=false
• 关键词: Dissertation; Research; Molecular; Systematics; Artemisia

9311086 Watson Artemesia ( sensu lato ) L. is composed of approximately 400 species distributed throughout the north temperate regions of the world. It is the largest and ecologically most important genus in the Tribe Anthemideae (Asteraceae). The genus has been the subject of numerous comparative biosystematic investigations, but remains poorly understood. Section Tridentatae is native to western North America and plays an important ecological role in the sagebrush communities. Tridentatae is composed of 11 species of xerophytic shrubs, separated from the other North American species by the presence of homogamous flower heads. The rapid expansion and divergence of Tridentatae , coupled with wind pollination has resulted in extensive convergence and parallel evolution. This has made interpretation of phylogenetic relationships difficult. The main objectives of this study are to elucidate interspecific phylogenetic relationships within Tridentatae , and to determine the affinity of Tridentatae to other subgeneric taxa of Artemesia and to the segregate genus Seriphidium . Several molecular techniques will be utilized including phylogenetic analysis of chloroplast DNA and nuclear ribosomal DNA restriction site maps and analysis of sequence data from the ORFk (Open Reading Frame k) region of the cpDNA genome. Previously proposed hypotheses of evolutionary relationships of Tridentatae will be tested using cladistic analyses of molecular data. An examination of concordance between cytoplasmic and nuclear marker based phylogenies of interspecific relationships will be used to test proposed modes of speciation and to examine patterns of character evolution. The inferred phylogenies will be useful in understanding the evolutionary and ecological success of Tridentatae in the cold deserts of western North America. %%% This project will obtain molecular data (both from the chloroplast and nuclear genomes) for 11 species of sagebrush in the Section Tridentatae of the genus Artemesia . These data will be used to decide relationships among these species that have been the subject of some confusion when morphological characters have been used to establish relationships. These woody shrubs are a highly visible and important component of the cold desert ecosystem and understanding species relationships will enhance other research efforts in ecology and biogeography of these plants. ***

9311086 Watson Artemesia（Sensu Lato）L。由大约400种分布在世界北部温带地区的物种组成。 它是部落国歌（Asteraceae）中最大，最重要的属。 该属一直是众多比较生物系统研究的主题，但仍然很少理解。 Tridentatae部分原产于北美西部，在鼠尾草社区中起着重要的生态作用。 Tridentatae由11种的三植物灌木组成，与其他同型花头的存在与其他北美物种分开。 三叉戟的快速膨胀和差异与风授粉相结合，导致了广泛的收敛和平行的进化。 这使得对系统发育关系的解释变得困难。 这项研究的主要目的是阐明三叉戟内的种间系统发育关系，并确定三叉戟与其他亚属亚氏菌和seiphidium属的亲和力。 将利用几种分子技术，包括叶绿体DNA和核核糖体DNA限制位点图的系统发育分析，以及来自CPDNA基因组的ORFK（开放式阅读框架K）区域的序列数据的分析。 先前提出的三叉戟进化关系的假设将使用分子数据分析进行测试。 研究种间关系的基于细胞质和核标记的系统发育之间的一致性将用于测试提出的物种形成模式并检查特征进化的模式。 推断的系统发育将有助于理解北美西部寒冷沙漠中三叉戟的进化和生态成功。该项目将在Artemesia属的三方段中获得11种鼠尾草的分子数据（来自叶绿体和核基因组）的分子数据（包括叶绿体和核基因组）。 这些数据将用于确定这些物种之间的关系，这些物种是在使用形态学特征来建立关系的时候是某种混乱的主题。 这些木质灌木是冷漠的生态系统中高度可见且重要的组成部分，了解物种关系将增强这些植物的生态学和生物地理学的其他研究工作。 ***