Arabidopsis DNA Methylation Mutants

拟南芥 DNA 甲基化突变体

• 批准号: 9306266
• 负责人: Eric Richards
• 金额: $ 34.5万
• 依托单位: Washington University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-08-01 至 1997-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9306266&HistoricalAwards=false
• 关键词: Arabidopsis; DNA; Methylation; Mutants

9306266 Richards Post-replicative methylation is the most common DNA modification present in eukaryotic cells. Although DNA methylation has been the subject of intense study, many questions remain about the function of this form of DNA modification in eukaryotes. The proposal outlines a genetic approach to the study of DNA methylation using the flowering plant Arabidopsis thaliana as a model. We have isolated A. thaliana mutants that contain reduced levels of cytosine methylation. Plans for further characterization of these hypomethylation mutants and strategies for isolating new methylation mutants are proposed. %%% It is anticipated that the A. thaliana mutants will provide a new system for studying eukaryotic DNA methylation. ***

9306266理查兹复制后甲基化是真核细胞中最常见的DNA修饰。 尽管DNA甲基化一直是激烈研究的主题，但关于这种DNA修饰的功能，真核生物中的许多问题仍然存在。 该提案概述了使用开花植物拟南芥作为模型进行DNA甲基化研究的遗传方法。 我们具有孤立的thaliana突变体，其中含有降低的胞嘧啶甲基化水平。 提出了进一步表征这些降压突变体的计划，并提出了隔离新甲基化突变体的策略。 %%%预计将为研究真核DNA甲基化提供新的系统。 ***