Purchase of a Fluorescence-Activated Cell Sorter

购买荧光激活细胞分选仪

• 批准号: 9217403
• 负责人: Robert Murphy
• 金额: $ 14.09万
• 依托单位: Carnegie-Mellon University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-02-15 至 1995-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9217403&HistoricalAwards=false
• 关键词: Purchase; Fluorescence; Activated; Cell; Sorter

This proposal is for the purchase of a fluorescence- activated cell sorter to replace one purchased in 1983 at the inception of the Center for Fluorescence Research in Biomedical Sciences at Carnegie Mellon University. The PI and co-PI are leaders in the flow cytometry community and the current FACS has been used in 38 research publications in cell biology in the 9 years since its installation. No other instrument exists at CMU. Limitations in its capabilities compared to newer instruments and difficulties with repairing and maintaining it justify replacement. The group of major and minor users receives over $3,290,000 in direct cost research support per year from federal and other agencies, including over $690,000 per year from NSF. The proposal replacement instrument will be used for research in a number of areas of cell, molecular and developmental biology, including membrane traffic, somatic cell genetics, fluorescent probe development, mitochondria physiology and cell differentiation and will be an integral part of the recently-established NSF Science and Technology Research Center in Light Microscopy Imaging and Biotechnology at Carnegie Mellon University

该提案是为了购买荧光激活的细胞分选蛋白，以取代1983年在卡内基·梅隆大学（Carnegie Mellon University）生物医学科学研究中心开始时购买的一个。 PI和CO-PI是流式细胞仪社区中的领导者，自安装以来的9年中，当前的FACS已在38个细胞生物学研究出版物中使用。 CMU没有其他乐器。 与较新的工具和维修和维护的困难相比，其功能的限制证明了替代的合理性。 一组主要用户和未成年人每年从联邦和其他机构获得超过3,290,000美元的直接成本研究支持，其中包括NSF的每年超过690,000美元。 该提案替换工具将用于在许多细胞，分子和发育生物学领域的研究中，包括膜交通，体细胞遗传学，荧光预处物发育，线粒体生理学和细胞分化，并将成为最近建立的NSF NSF科学和技术研究中心的一部分