Application for a Fluorescence-Activated Cell Sorter

荧光激活细胞分选仪的应用

• 批准号: 6580515
• 负责人: ELLEN V. ROTHENBERG
• 金额: $ 50万
• 依托单位: CALIFORNIA INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-06-01 至 2004-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6580515
• 关键词: biomedical equipment purchase; flow cytometry; biomedical equipment purchase; flow cytometry

DESCRIPTION (provided by applicant): This application is to buy a new, high-performance fluorescence-activated cell sorter to serve a diverse group of clients at the California Institute of Technology Flow Cytometry/Cell Sorting Facility. The user group currently served by the Facility includes not only immunologists, but also developmental neurobiologists, genomics experts, structural biochemists, and chemical engineers using bacterial populations to model evolutionary logic. This range of research demands many distinct capabilities of a cell sorter, including the ability to sort rare populations quickly, robust multicolor analysis, the ability to sort bacteria and irregularly shaped cells, and the ability to excite and detect fluorescence resonance energy transfer between genetically engineered fusion proteins for tests of protein-protein interactions within live cells. These diverse applications call for a high-performance, modern cell sorter, and some of them cannot be served by any existing Facility instrumentation. The Facility presently owns two cell sorters and an analyzer, but one of the sorters is over 13 years old and incapable of providing important services. The difficulty of obtaining sorting time has become a major constraint on experimental progress. This application is to replace the old sorter with a new sorter that will improve service to existing clients and permit the Facility to carry out new flow cytometric applications that have not been available to the user group before.

描述（由申请人提供）：此申请是购买一种新的高性能荧光激活的细胞分选项，以服务于加利福尼亚技术学院流式细胞仪/细胞分类设施的一群客户。该设施目前服务的用户组不仅包括免疫学家，还包括发展性神经生物学家，基因组学专家，结构生物化学家和化学工程师，使用细菌种群来模拟进化逻辑。这项研究范围需要细胞分类器的许多不同功能，包括快速分类稀有种群的能力，可靠的多色分析，分类细菌和不规则形状的细胞的能力，以及能够激发和检测荧光共振能量转移的能力，以在基因工程融合蛋白之间进行蛋白质蛋白质相互作用的活细胞中的基因工程融合蛋白之间的能力。这些多样化的应用要求高性能，现代的细胞分辨率，其中一些无法由任何现有的设施仪器提供服务。该设施目前拥有两个细胞分类器和一个分析仪，但其中一名分类器已有13年的历史，无法提供重要的服务。获得排序时间的困难已成为对实验进步的主要限制。该应用程序是用一个新的分类器替换旧的分类器，该分类器将改善对现有客户的服务，并允许该设施执行以前尚未为用户组可用的新流式细胞仪应用程序。