Fluorescence-Activated Cell Sorter

荧光激活细胞分选仪

• 批准号: 6735220
• 负责人: CHRISTIAN W SCHINDLER
• 金额: $ 32.84万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6735220
• 关键词: biomedical equipment purchase; flow cytometry

DESCRIPTION (provided by applicant): Flow cytometry enables investigators to identify, purify and characterize cells from mixed populations, based on a pattern of proteins they express. At Columbia University, like most other institutions, the growing need to carry out studies in primary cells has made flow cytometry an increasingly essential tool for biomedical research. These institutional needs are in large part met by the Columbia University Flow Cytometry Core Facility, which currently operates two analytical cytometers (a FACS Calibur and FACScan) and a heavily modified FACStar Plus cell sorter. The sorter, originally purchased in 1991 through a Shared Instrumentation Grant, despite modifications, has reached the limit of its useful life. Moreover, with the development of the new FACS cell-sorting platform, support for the FACStar Plus platform is becoming more difficult. In this proposal we request funding to replace our FACStar Plus with a Becton-Dickinson (B-D) FACS Aria cell sorter. We propose to equip this sorter in its standard 3-laser configuration, providing it with all the features we require to carry out state-of-the-art accurate high-speed multi-parameter cell sorting. Additional support, for both the purchase and operation of this cell sorter will continue to be provided by Columbia University Cancer Center and user fees. Replacing our aging FACStar Plus with a modem FACS Aria cell sorter will not only enable the research community at Columbia University to remain competitive in several areas of biomedical research, but will also facilitate our ability to retain and recruit faculty, especially in the field of immunology.

描述（由申请人提供）：流式细胞仪使研究人员能够根据其表达的蛋白质模式来识别，净化和表征混合种群中的细胞。与大多数其他机构一样，在哥伦比亚大学，在原始细胞中进行研究的需求日益增长，使流式细胞术成为生物医学研究的越来越重要的工具。这些机构需求在很大程度上由哥伦比亚大学流式细胞仪核心设施满足，该机构目前运营两个分析细胞仪（FACS Calibur和FACSCAN）和一个重大修改的FACSTAR PLES PLES分类器。该分散者最初通过共享的仪器赠款于1991年购买，尽管进行了修改，但仍达到了其使用寿命的极限。此外，随着新FACS细胞分类平台的开发，Facstar Plus平台的支持变得越来越困难。 在此提案中，我们要求资金用Becton-Dickinson（B-D）FACS ARIA Cell Sorter代替Facstar Plus。我们建议将该分类器以其标准的3射线配置装备，从而为其提供我们所需的所有功能，以执行最先进的准确的高速多参数细胞分类。哥伦比亚大学癌症中心和用户费用将继续提供此细胞分隔器的购买和操作的额外支持。用调制解调器FACS ARIA细胞分类器代替老化的Facstar Plus不仅会使哥伦比亚大学的研究界能够在生物医学研究的几个领域保持竞争力，而且还将促进我们保留和招募教师的能力，尤其是在免疫学领域。