Molecular Evaluation of Disease Resistance and Susceptibility in Oats

燕麦抗病性和易感性的分子评价

• 批准号: 9018571
• 负责人: Thomas Wolpert
• 金额: $ 15万
• 依托单位: Oregon State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-03-15 至 1994-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9018571&HistoricalAwards=false
• 关键词: Molecular; Evaluation; Disease; Resistance; Susceptibility

This proposal is directed at evaluating whether a victorin-binding protein (VBP) isolated from oat is the receptor for this toxin and is the molecular determinant of susceptibility to the disease Victoria bight. Using purified VBP, antibody thereto, and a full length cDNA already cloned, the functional role of VBP will be examined in biochemical aspects, the binding domains examined, sequence of VBP-cDNA determined, a determination of segregation of the gene encoding VBP studied, and sequence heterogeneity in VBP of resistant and susceptible phenotypes compared. This work is directed at a critical issue in modern plant pathology, namely an understanding of the site and function of a plant gene product which regulated the pathogenicity of a fungal pathogen that causes the disease, Victoria bight, of oat.

该提案旨在评估从燕麦中分离出的维克多林结合蛋白（VBP）是否是该毒素的受体，以及是否是维多利亚湾病易感性的分子决定因素。 使用纯化的VBP、其抗体和已经克隆的全长cDNA，将在生化方面检查VBP的功能作用，检查结合结构域，确定VBP-cDNA的序列，确定所研究的编码VBP的基因的分离，比较抗性和易感表型的 VBP 序列异质性。 这项工作针对现代植物病理学中的一个关键问题，即了解植物基因产物的位点和功能，该基因产物调节引起燕麦维多利亚病的真菌病原体的致病性。