Identification of the Vb Gene Which Confers Sensitivity to the Host-Selective Toxin, Victorin

鉴定赋予对宿主选择性毒素 Victorin 敏感性的 Vb 基因

基本信息

批准号：
9631442
负责人：
Thomas Wolpert
金额：
$ 30万
依托单位：
Oregon State University
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
1996
资助国家：
美国
起止时间：
1996-09-01 至 2000-08-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9631442&HistoricalAwards=false
关键词：
Identification Vb Gene Confers Sensitivity

项目摘要

9631442 Wolpert Victoria blight of oats is caused by the fungus Cochliobolus victoriae. This fungus is pathogenic because it produces the host-specific toxin, victorin. In the host, sensitivity to victorin, and thus, susceptibility to the pathogen, is conditioned by the dominant allele at the Vb locus. It has been hypothesized that the Vb gene conditions susceptibility by coding for a toxin "receptor" (site of action) which is functional in toxin-sensitive lines (susceptible lines) and either absent or altered in toxin-insensitive lines. Biologically-active, labeled derivatives of the toxin have been used to identify and 100 kDa protein that binds victorin only in toxin-sensitive (susceptible) genotypes of oats and a 15 kDa protein that binds victorin in both susceptible and resistant genotypes. The 100 kDa victorin-binding protein (VBP) has been identified as the P-protein component of the nuclear-encoded, mitochondrial multienzyme complex, glycine decarboxylase. Results have also indicated that the 15 kDa H-protein component of the glycine decarboxylase complex (GDC) is a VBP. This research will test a biochemical model that toxin specificity is regulated by the interaction of the two victorin binding proteins, the P- and the H-protein components of the GDC. The results will provide new information about the nature of Host-specific toxin dependent plant disease, which may lead to a new crop protection strategy.

9631442燕麦的沃尔珀特维多利亚疫苗是由真菌Cochliobolus Victoriae引起的。这种真菌具有致病性，因为它会产生特定于宿主的毒素Victorin。在宿主中，对维多林的敏感性，因此对病原体的敏感性，由VB基因座的主要等位基因调节。据推测，VB基因通过编码毒素“受体”（作用部位）来敏感，该毒素“受体”（作用部位）在毒素敏感的线（易感线）中起作用，并且在毒素不敏感的线中不存在或不变。毒素的生物活性，标记的标记衍生物已用于鉴定仅在燕麦的毒素敏感（易感性）基因型中结合维多林的100 kDa蛋白，并结合15 kDa蛋白，该蛋白结合了易感性和抗药性基因型中的维多林。 100 kDa维多林结合蛋白（VBP）已被确定为核编码，线粒体多酶复合物，甘氨酸脱羧酶的P蛋白成分。结果还表明，甘氨酸脱羧酶复合物（GDC）的15 kDa H蛋白成分是VBP。这项研究将测试一个生化模型，即毒素特异性受到GDC的P-和H-蛋白成分的相互作用来调节毒素特异性。结果将提供有关宿主特异性毒素依赖植物性植物疾病性质的新信息，这可能会导致新的作物保护策略。