Molecular Mechanisms of Sodium Channel Sorting in Neurons

神经元钠通道排序的分子机制

• 批准号: 9006436
• 负责人: William Gilly
• 金额: $ 1.67万
• 依托单位: Stanford University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-07-01 至 1991-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9006436&HistoricalAwards=false
• 关键词: Molecular; Mechanisms; Sodium; Channel; Sorting

Nerve cell activity depends on various ions crossing the cell membrane through channels that are sometimes very specific. These channels are formed by protein molecules in the membrane. Nerve cells often have a long process, the axon which extends from the cell body known as the soma. Often channels in the axon are distributed differently from those in the soma. This research addresses the question of how different proteins, which are continuously synthesized in the soma of nerve cells, are spatially distributed. This project will examine the molecular mechanisms of sorting sodium channel proteins in nerve cells. Sodium channel proteins are synthesized in the soma, but are found in the membrane of the axon and are absent in the soma. In the squid, an invertebrate, mRNA coding from rat sodium channels will be injected into the soma. As a result the cells will synthesize two different types of sodium channels making it possible to compare the spatial distribution of the two protein molecules. The part of the molecule responsible for its peculiar spatial distribution can be changed by changing the coding and thus is able to be identified. This study will advance our understanding of long-term regulation of how nerve cells function.

神经细胞活性取决于各种有时非常具体的通道越过细胞膜的各种离子。 这些通道是由膜中蛋白质分子形成的。 神经细胞通常具有较长的过程，轴突从称为SOMA的细胞体延伸。 轴突中的通道通常与躯体中的通道分布不同。 这项研究解决了一个问题，即在神经细胞的躯体中连续合成的不同蛋白质如何在空间上分布。 该项目将检查神经细胞中钠通道蛋白的分子机制。 钠通道蛋白是在躯体中合成的，但在轴突的膜中发现，在体内不存在。在鱿鱼中，无脊椎动物，将从大鼠钠通道中编码的mRNA编码将被注入体内。 结果，细胞将合成两种不同类型的钠通道，从而可以比较两个蛋白质分子的空间分布。 负责其特殊空间分布的分子的一部分可以通过更改编码来更改，因此可以识别。 这项研究将促进我们对神经细胞的长期调节的理解。