Structure and Function of a Novel Biotin Enzyme

新型生物素酶的结构和功能

基本信息

批准号：
9004409
负责人：
Basil Nikolau
金额：
$ 14万
依托单位：
Iowa State University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
1990
资助国家：
美国
起止时间：
1990-08-01 至 1993-01-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9004409&HistoricalAwards=false
关键词：
Structure Function Novel Biotin Enzyme

项目摘要

This research focuses on understanding the significance and role of biotin enzymes in plant metabolism. To achieve this goal the unique somatic embryogenesis system of carrot has been utilized to study these proteins. During the development of somatic embryos of carrot the steady state levels of one of the six biotin-containing polypeptides increases dramatically. This biotin protein has been purified and polyclonal antibodies against it have been prepared. Using these antibodies a cDNA clone coding for this biotin protein has been isolated. This novel biotin protein is being investigated through the utilization of these antibodies and the cDNA clone as specific probes to investigate this novel biotin protein. These investigations concentrate on elucidating the function and regulation of this biotin enzyme. Initial experiments with the antibodies have identified this biotin protein as representing an acetyl CoA carboxylase. The function of this form of acetyl-CoA carboxylase in plant metabolism is being elucidated by 1) identifying the subcellular and tissue distribution of this protein in the carrot plant and 2) elucidating the molecular genetic mechanisms that regulate the accumulation of this protein in response to developmental and environmental signals. Biotin is an essential component of a set of enzymes that have important metabolic functions. The structure, function and regulation of a number of biotin enzymes is extensively studied in a diverse set of organisms. However, very little is known about the biotin enzymes of plants. This research investigates the function and regulation of plant biotin- containing enzymes in order to provide a better understanding of their role in plant metabolism.***//

这项研究重点是了解生物素酶在植物代谢中的重要性和作用。为了实现这一目标，已经利用了独特的胡萝卜的体细胞胚发生系统来研究这些蛋白质。在胡萝卜的体细胞胚胎的发展过程中，六个含生物素多肽之一的稳态水平急剧增加。该生物素蛋白已被纯化，并且已经制备了针对其的多克隆抗体。使用这些抗体，已经分离了该生物素蛋白的cDNA克隆编码。通过利用这些抗体和cDNA克隆作为研究这种新型生物素蛋白的特定探针，正在研究这种新型的生物素蛋白。这些研究集中于阐明该生物素酶的功能和调节。使用抗体的初始实验已将这种生物素蛋白鉴定为代表乙酰COA羧化酶。这种形式的乙酰-COA羧化酶在植物代谢中的功能是通过1）识别胡萝卜植物中该蛋白的亚细胞和组织分布的阐明，2）阐明了该蛋白质的分子遗传机制，以调节该蛋白的积累，以响应该蛋白发展和环境信号。生物素是具有重要代谢功能的一组酶的重要组成部分。许多生物素酶的结构，功能和调节在一组生物体中进行了广泛的研究。但是，关于植物的生物素酶知之甚少。这项研究调查了含有植物生物素的酶的功能和调节，以便更好地了解其在植物代谢中的作用。*** //