Research Initiation Award: Investigation of the Effects of Dissolved Oxygen and Dissolved Carbon Dioxide on Embryogenesis and the Competence for Embryogenesis

研究启动奖：溶解氧和溶解二氧化碳对胚胎发生的影响和胚胎发生能力的研究

• 批准号: 8707827
• 负责人: Gregory Payne
• 金额: $ 6万
• 依托单位: University of Maryland Baltimore County
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-07-01 至 1989-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8707827&HistoricalAwards=false
• 关键词: Research; Initiation; Award; Investigation; Effects

Narrative: The somatic embryogenesis of carrots will be used as a model to quantify the effects of dissolved oxygen and dissolved carbon dioxide on undifferentiated cell growth and embryogenesis. This model has been selected because extensive information regarding somatic embryogenesis of carrots already exists in the literature. Embryos are thought to originate from single cells, or a small number of cells associated with a cluster of cells. After induction, the embryos progress through several stages of development. Recognition of the various stages of embryogenesis and plantlet regeneration will comprise the major analytical procedures for assessing embryogenesis for this project. Gases of various composition will be used in shake flask studies and the information will then be transferred to the more industrially applicable system of an agitated, scalable reactor. The oxygen and carbon dioxide effects will be studied to develop a bioreactor that can be operated at controlled levels of the gaseous components. This development will provide a valuable tool for further investigations that will require the cultivation of embryos under well defined nutritional conditions.

叙述：胡萝卜的体细胞胚胎发生将被用作量化溶解氧和溶解二氧化碳对未分化细胞生长和胚胎发生的影响的模型。 选择该模型是因为文献中已经存在有关胡萝卜体细胞胚胎发生的大量信息。 胚胎被认为起源于单个细胞或与细胞簇相关的少量细胞。 诱导后，胚胎会经历几个发育阶段。 对胚胎发生和植株再生的各个阶段的识别将构成评估该项目胚胎发生的主要分析程序。 各种成分的气体将用于摇瓶研究，然后信息将被转移到工业上更适用的搅拌式可扩展反应器系统中。 将研究氧气和二氧化碳的影响，以开发一种可以在受控气体成分水平下运行的生物反应器。 这一进展将为进一步研究提供一个有价值的工具，这些研究需要在明确的营养条件下培养胚胎。