基于核酸-光/电传导界面的均相电致化学发光生物传感及细胞成像研究

In this project, ultrasensitive homogeneous electrochemiluminescence biosensing and cell imaging will be developed based on DNA-photic/electric transduce interface. According to various principle of electrochemiluminescent reaction, inorganic and organic single-strand DNA-sensitive membranes are fabricated, respectively, using Layered 2D nanomaterials and conductive block copolymers to construct affinity-mediated homogeneous electrochemiluminescence biosensing electrodes, which enhanced the discrimination ability of electrode over single-stranded DNA and double-stranded DNA. Then electrochemiluminescent single-strand DNA-sensitive organic membrane with low excitation potential and fast electrochemiluminescent response is constructed through the internal in-situ fabrication of nanoclusters or conjugated small molecules as electrochemiluminescent luminophors. Then, ultrasensitive and single-molecular homogeneous biosensing method will be developed with the aid of novel homogeneous signal amplification strategies. According to the photovoltaic principle of metalloid semiconductors, an enhancing mechanism of photic-activated electric-photic signal feedback oscillation will be developed to construct dual-electrode homogeneous electrochemiluminescence sandwich cell imaging strategy, resulting high resolution, ultrasensitive, and single-cell homogeneous electrochemiluminescence imaging array. This project is of great scientific importance, and innovative research results are expected.

本项目拟开展基于核酸-光/电传导界面的超灵敏均相电致化学发光生物传感及细胞成像研究。根据不同的电致化学发光原理，利用层状二维纳米材料与导电嵌段共聚物，开发单链DNA无机/有机敏感膜，构建亲和力介导均相电致化学发光生物传感电极，提升电极对单/双链DNA的特异性区分能力。探索电致化学发光纳米簇与共轭小分子发光体的原位制备技术，构筑响应速度快、激发电位低的共反应式电致化学发光单链DNA有机敏感膜。设计均相电致化学发光信号放大新策略，实现超灵敏、单分子均相电致化学发光生物传感。根据非金属半导体光伏原理，研究探索光信号-激活电信号-光信号反馈振荡增强新机制，发展双电极夹心式均相电致化学发光细胞成像新策略，为实现超灵敏、单细胞、阵列化、高空间分辨的均相电致化学发光成像分析奠定理论与技术基础，具有重要的科学意义和研究价值，有望获得一系列具有特色的创新性研究成果。