纳米材料功能化针灸针的研制及其在穴实时监测五羟色胺

Acupuncture, as a bright and dazzling pearl in Chinese medical science, has made great contribution to the multiplication and prosperity of the Chinese nation and the human health care for thousands of years. Acupuncture and moxibustion is a treatment, in which a needle is utilized to stimulate human body and make effects. However, its causes are still an open question. This project for the first time combines modern nanotechnology with traditional acupuncture needle to fabricate nanomaterial-functionalized acupuncture needle capable of in vivo and real time monitoring serotonin (5-HT) in local acupoints caused by acupuncture. Various strategies are employed to modify different nanomaterials to the tip surface of acupuncture needle, resulting in nanomaterial functionalized acupuncture needle having the properties of good stability, strong anti-interference ability and high sensitivity. The sensing performance of the nanoscale acupuncture needle is investigated, and in vivo and non-destructive measurement is carried out with stable and real signal. A new method of detecting active biomolecules based on nanoscale acupuncture needle is constructed. Meanwhile, a living detection system based on the nanoscale acupuncture needle is also established, which is able to monitor 5-HT in local acupoints in an in vivo and real time manner. In addition, an immunohistochemistry is utilized to study kinetic change of histocytochemistry in acupoint tissues before and after acupuncture, which can provide evidence to support the conclusion of the above-mentioned method of using nanomaterial functionalized acupuncture needle. The establishment of this project will help reveal the mechanism of acupuncture treatment, making a good basis for international development of acupuncture needle.

针灸医学作为中国医药学宝库中一颗璀璨夺目的明珠，几千年来为中华民族的繁衍昌盛和人类的保健事业作出了巨大贡献。针灸是一种用针刺激人体产生效果的治疗手段，其成因还有待研究。本项目将纳米技术与传统针灸技术融合，构建纳米材料功能化的针灸传感针实时在体监测由针刺引起的穴位局部五羟色胺浓度动态变化。采用不同的策略将不同纳米材料固定在针灸针表面，得到稳定性好、抗干扰能力强及灵敏度高的纳米材料功能化针灸传感针。考察纳米针灸传感针的传感性能，实现无损在体测量，采集的信号稳定、真实。建立基于纳米材料功能化针灸传感针检测生物信号分子的新方法，确立纳米针灸传感针在体监测穴位局部五羟色胺浓度动态变化的活体检测系统。与此同时，利用免疫组织化学的方法研究针刺前后穴位周围组织细胞化学的动态变化，对电化学检测方法进行有效佐证。本项目的确立将有助于揭示针灸治病的机理，为针灸的国际化发展打下基础。

针灸治疗疾病具有较好的疗效，但其作用机理尚不明确。本项目首次将纳米技术与传统针灸技术融合，构建纳米材料修饰的针灸传感针实时在体监测由针刺引起的穴位局部五羟色胺浓度动态变化。用绝缘层将针灸针基体部分进行绝缘，分别采用不同修饰方法将不同纳米材料修饰到针灸针针尖表面，得到纳米材料功能化的针灸传感针。考察纳米针灸传感针的稳定性，并实现无损在体测量，采集的信号稳定、真实。建立基于纳米针灸传感针检测五羟色胺的新方法，实现纳米针灸传感针在体监测穴位局部五羟色胺浓度动态变化的活体检测系统。与此同时，利用免疫组织化学的方法研究针刺前后穴位组织细胞化学的动态变化，从而对电化学检测方法进行有效佐证。主要研究内容：1）研制了碳纳米管修饰的针灸针用于大鼠活体五羟色胺的实时监测。2）研制了卟啉石墨烯功能化纳米针灸传感针活体在穴实时监测一氧化氮。3）开发了二硫化钼/铂纳米颗粒功能化针灸传感针用于细胞水平过氧化氢的实时监测。4）研制了二硫化钼/聚苯胺功能化针灸传感针用于活体脑脊液pH的实时监测。5）开发了金纳米线/碳纳米管功能化针灸传感针用于大鼠脑蓝斑核去甲肾上腺素实时监测。 6）针型场效应晶体管生物传感器的研制。本项目的确立将有助于揭示针灸治病的机理，为针灸的国际化发展打下基础。

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