长期锻炼作用下早期生长因子Egr-1介导骨骼肌细胞糖脂代谢模式变化及分泌以调控脂肪细胞脂质积累的机制研究

Long-term exercise can effectively improve the body's metabolic state and prevent the occurrence of chronic metabolic diseases. The metabolic patterns of skeletal muscle and adipose tissue alters significantly under long-term exercise through the cross-talking each other, but the mechanism is still unclear. Our previous study found that long-term exercise can increase the expression of a transcription factor early growth response factor (Egr-1). Further examination showed that specific knockout Egr-1 in skeletal muscle resulted in the abnormal metabolism patterns in skeletal muscle and increased lipid accumulation in adipose tissue. This project intends to explore how Egr-1 in muscle cells regulate the expression and secretion of skeletal muscle-specific genes, affecting the function of adipose tissue under long-term exercise. We will reveal the metabolic communication mechanism between skeletal muscle and adipose tissue under long-term exercise. Our study would make us to know how the excise effectively improves the body's metabolic capacity, and provide a new perspective for the prevention of chronic metabolic diseases.

长期锻炼能够有效改善机体代谢状态，预防慢性代谢疾病的发生。骨骼肌和脂肪组织在长期锻炼作用下代谢模式均发生显著性变化，并通过释放特定因子相互影响对方的生理功能。但骨骼肌代谢模式的改变以及影响脂肪组织功能的作用机制尚不明确。我们前期研究发现长期锻炼作用可导致转录因子早期生长反应因子Egr-1表达水平增加；骨骼肌细胞特异性敲除Egr-1小鼠的肌肉代谢模式异常，同时外周脂肪组织的脂质积累增加。因此我们认为在长期锻炼作用下Egr-1对骨骼肌代谢和与脂肪组织的相互交流发挥着重要的作用。本项目拟利用骨骼肌细胞特异性敲除Egr-1小鼠模型，探讨长期锻炼作用下，骨骼肌细胞Egr-1通过调控代谢相关基因或特定因子的表达和分泌，影响脂肪细胞脂质积累的作用方式及分子机制，揭示长期锻炼作用下肌肉细胞与脂肪组织细胞的代谢交流机制。为通过锻炼有效改善机体的代谢能力，预防慢性代谢疾病提供一个新的角度。