EB1的翻译后修饰对细胞行为的影响

EB1 is a microtubule binding protein, which can regulate microtubule dynamics, and is related with many kinds of cell activities, even with occurence of cancer and other diseases. The expression of EB1 protein is stable, and the post-translational modification of EB1 can be involved in the regulation of EB1 function and the influence on the cell activity and disease..The research contents of this project, will be based on the previous work of our group. First, we will detect the post-modifications of EB1, suchas ubiquitination, acetylation and so on. Secondly, wie will analyse the role of EB1 modification on its regulation of microtubule dynamics, spindle behavior, and other proteins binding to microtubules. Thirdly, based on the modification of EB1 on the cell activity, we will study the effects of EB1 modification on the important process of cell activity, such as cell movement, cell division and differentiation. Finally, we will also investigaye the relationship between EB1 modificationthe and development of tumor and related diseases..The results of these studies will help to identify the regulatory function of EB1 post-translational modification, which can provide a theoretical basis for understanding function of EB1. At the same time, the results also can contribute to further understanding of EB1 in tumorigenesis and provide new theoretical basis for the clinical treatment of the disease.

EB作为是微管末端结合蛋白，可调控微管动态性，并与多种细胞活动，以及肿瘤等疾病的发生有密切联系。EB1蛋白表达稳定，蛋白的翻译后修饰可调节EB1蛋白功能，并参与对细胞活动和疾病发生的影响。.本项目的研究内容，将在课题组前期工作的基础上，论证EB1发生泛素化、乙酰化等修饰作用的具体方式；检测EB1的修饰对微管动态性，微管相关蛋白结合微管能力等方面的调节机制；在此基础上，研究EB1的修饰对相关细胞活性，如细胞运动、细胞分裂、分化等过程的影响和作用方式；最后，还将就EB1的修饰作用在肿瘤等相关疾病的发生、发展中的作用等进行探索。.预期的研究成果将有助于加深对EB1翻译后修饰的调控功能的理解，为解析EB1的生理功能提供理论依据；同时也有助于深入解读EB1在肿瘤等疾病发生中的作用，为疾病的临床治疗提供新的理论依据。

EB1 蛋白作为微管末端结合蛋白，通过结合微管以及介导 Tips 结合微管，可调节微管动态性，并参与多项重要的生理活动，如，细胞运动，纺锤体定向等。 . EB1 蛋白表达水平稳定，其可通过自身的多种翻译后修饰作用，调节 EB1 蛋白的构象和功能，进而参与对细胞活动的影响。 . 本项目围绕 EB1 蛋白的磷酸化、乙酰化、谷胱甘肽化等翻译后修饰作用展开研究工作，明确 EB1 蛋白发生的修饰作用类型和作用位点；并探究修饰作用调控EB1的相关活性的方式，鉴定对细胞活性的影响，探讨关联的生理活性和相关疾病。. 发现EB1的苏氨酸和酪氨酸的磷酸化修饰作用，可通过对微管结构和动态性的调节，影响纺锤体组装，定向和细胞分裂进程，并能影响胰腺癌发展。. 发现EB1的乙酰化修饰作用，确定新的EB1乙酰化位点，发现该位点的乙酰化加速了自噬介导的EB1降解，并进而调节微管动态结构。. 发现EB1的谷胱甘肽化修饰，该修饰作用可影响微管动态性，并参与调控相关的细胞活性，导致细胞分裂方向和细胞迁移缺陷。

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