Instrument to recapture and handle VOCs

回收和处理挥发性有机化合物的仪器

• 批准号: RTI-2023-00171
• 负责人: Hill, Jane
• 金额: $ 10.57万
• 依托单位: University of British Columbia
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=746774
• 关键词: Instrument; recapture; handle; VOCs

The Hill lab specializes in untargeted (i.e., discovery) metabolomics for VOCs produced by plants, microorganisms, and mammals. We typically use thermal desorption tubes (TDTs) for the collection and concentration of VOCs and analyze them comprehensively on state-of-the-art analytical instruments. However, very often, we can only collect one sample per sampling point. Therefore, due to the untargeted nature of our work, when we only have one sample per time point or sampling point, we cannot run our chromatography in a fully optimized manner. If we had the ability to take that one sample and create technical replicates from it, we would be able to optimize the analysis. In addition, having replicates would allow us us to spike in validation standards on replicate samples to confirm compounds or to quantify them as well as afford us the ability to preserve replicate samples in a bank for future analysis. We can solve our "one precious sample" problem via purchase of a Recollect 10TM instrument. This system generates up to 10 technical replicates of the sample, distributing one sample equally across all tubes or in a dilution series. The requested instrument is the only stand-alone instrument available commercially that can generate replicates or dilution series without the need to commandeer an analytical instrument. We also require a robot system to run the recollected TDTs on the analytical system as the TDTs used in the Recollect 10T are of a geometry and material (steel) that will not work on our current robot. We have selected the TD 100-xrTM robot, which is an automated thermal desorption system capable of rapid processing of up to 100 TDTs in a single sequence and is compatible with the TDTs. The use of these two systems will substantially improve the quality of our scientific work. We have confirmed from the instrument manufacturers that no Recollect 10TM instrument is present on the UBC campus nor elsewhere in British Columbia. The same is true of other instruments capable of recollecting TDT content. We anticipate that 18 HQP, including postdoctoral research fellows, graduate students and technical staff, will be trained using the requested equipment over the next 5 years. Our diverse team of HQP will acquire technical and hands-on-training while working with the requested instrument that will enhance their competitiveness for the biomedical and analytical positions.

Hill 实验室专门研究植物、微生物和哺乳动物产生的 VOC 的非靶向（即发现）代谢组学。我们通常使用热脱附管 (TDT) 来收集和浓缩 VOC，并在最先进的分析仪器上对其进行全面分析。然而，很多时候，我们每个采样点只能采集一个样本。因此，由于我们工作的非目标性，当我们每个时间点或采样点只有一个样品时，我们无法以完全优化的方式运行色谱。如果我们有能力获取该样本并从中创建技术复制品，我们就能够优化分析。此外，拥有重复样本将使我们能够提高重复样本的验证标准，以确认化合物或量化它们，并使我们能够将重复样本保存在银行中以供将来分析。我们可以通过购买 Recollect 10TM 仪器来解决“一个珍贵样品”的问题。该系统可生成多达 10 个样品的技术复制品，将一份样品均匀地分布在所有管中或稀释系列中。所要求的仪器是唯一可商用的独立仪器，可以生成重复或稀释系列，而无需征用分析仪器。我们还需要一个机器人系统在分析系统上运行重新收集的 TDT，因为 Recollect 10T 中使用的 TDT 的几何形状和材料（钢）不适用于我们当前的机器人。我们选择了 TD 100-xrTM 机器人，这是一种自动化热解吸系统，能够在单个序列中快速处理多达 100 个 TDT，并且与 TDT 兼容。这两个系统的使用将大大提高我们科学工作的质量。 我们已从仪器制造商处确认，UBC 校园或不列颠哥伦比亚省其他地方没有 Recollect 10TM 仪器。其他能够回忆 TDT 内容的仪器也是如此。 我们预计未来 5 年内将使用所需设备对 18 名 HQP（包括博士后研究员、研究生和技术人员）进行培训。我们多元化的 HQP 团队将在使用所需仪器的同时获得技术和实践培训，这将增强他们在生物医学和分析职位上的竞争力。