From Symbiosis to Methanotrophy: Diverse Functions in Rhizobiales

从共生到甲烷氧化：根瘤菌的多种功能

• 批准号: RGPIN-2018-04754
• 负责人: Charles, Trevor
• 金额: $ 6.12万
• 依托单位: University of Waterloo
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=745643
• 关键词: Symbiosis; Methanotrophy; Diverse; Functions; Rhizobiales

Our NSERC Discovery program has revolved around Sinorhizobium meliloti, the nitrogen fixing symbiont of alfalfa, since 1995. This has served as the foundation for all other work in our lab. With this proposal, we are continuing to work in S. meliloti, investigating genetic control of root nodule infection, and also regulatory links between metabolism of PHB, which is the major intracellular carbon store, and nitrogen metabolism. We will also extend our research to another member of the order Rhizobiales, Methylosinus trichosporium strain OB3b, which we started working on a few years ago. This strain is a methanotroph, which has a restricted carbon utilization capability, limited to C1 compounds such as methane and methanol. We will investigate the role of PHB in methanotrophic growth, and initiate research towards defining the minimal methanotrophic genome. The work in M. trichosporium will result in the development and refinement of genome engineering methods for this important methanotroph, pave the way for development of specialized strains for the conversion of methane to high value bioproducts, and provide insight into the genetic and metabolic requirements for methanotrophy.

自1995年以来，我们的NSERC发现计划围绕着苜蓿的氮固定共生体Sinorhizobium Meliloti。这是我们实验室中所有其他工作的基础。有了这一建议，我们将继续在梅洛蒂（S. s. s. s.s。meliloti）中工作，研究根结节感染的遗传控制，以及PHB代谢之间的调节性联系，PHB是主要的细胞内碳储存储存和氮代谢。我们还将将研究扩展到几年前我们开始从事的另一个Rhizobiales甲基孢子菌株OB3B甲基孢子菌素菌株OB3B。该菌株是一种甲烷营养物，具有受限的碳利用能力，仅限于甲烷和甲醇等C1化合物。我们将研究PHB在甲嗜酸性生长中的作用，并开始研究定义最小的甲状腺营养基因组。 trichosporium开发的工作将导致这种重要的甲烷植物的基因组工程方法的发展和完善，为开发专业菌株的发展铺平了甲烷转化为高价值生物生物生物生物生物生物生物生物生物产品的道路，并洞悉甲烷的遗传和代谢需求。