Determining spatial arrangement, stoichiometry, and substrate specificity of messenger RNA-binding proteins along the gene expression pathway.

确定基因表达途径中信使 RNA 结合蛋白的空间排列、化学计量和底物特异性。

• 批准号: RGPIN-2020-06924
• 负责人: Oeffinger, Marlene
• 金额: $ 3.06万
• 依托单位: Université de Montréal
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=744382
• 关键词: Determining; spatial; arrangement; stoichiometry; substrate

The long-term objective of our research program is to study the maturation pathways of essential RNA-protein complexes in different organisms. The expression of our genes is tightly regulated both during the reading and copying of our DNA into messenger RNA (mRNA), the encoding template, and during translation, when the information in this template is converted into proteins. However, transcription and translation are not the only steps where regulation and alteration of our genetic material can occur. mRNA itself is bound by many proteins that further process, fold and inspect the molecule to ensure its correctness and hence the correct transmission of genetic code into proteins for diverse processes in the cell. While it has been assumed that all mRNA molecules are uniformly processed, or matured, by the same set of proteins, recent data from our lab suggests that there may be variation in these maturation steps that are defined by the variance in proteins associating with different mRNAs during these steps. This project proposes to further explore these suggested variances in mRNA maturation, by investigating the mRNAs involved, the underlying mechanisms that lead to variances, and their consequences, as any misstep in this process could lead to the introduction of errors resulting in disease. Overall, a more detailed basic knowledge of any additional regulatory steps during the expression of our genetic material will provide us with better fundamental understanding of gene regulation as well as why certain diseases may develop and potential new entry points for treatment in the future.

我们研究计划的长期目标是研究不同生物体中必需的RNA蛋白质复合物的成熟途径。在将DNA读取和复制到信使RNA（mRNA），编码模板和翻译过程中，当该模板中的信息转换为蛋白质时，我们的基因的表达受到了严格的调节。但是，转录和翻译并不是可能发生调节和改变我们遗传物质的唯一步骤。 mRNA本身受许多蛋白质的约束，这些蛋白质会进一步进行，折叠和检查分子，以确保其正确性，从而将遗传密码正确地传递到细胞中的潜水过程中。尽管已经假定所有mRNA分子都是通过相同的蛋白质均匀处理或成熟的，但我们实验室的最新数据表明，这些成熟步骤可能存在因素在这些步骤中与不同mRNA相关的蛋白质方差所定义的变化。该项目的建议是通过研究所涉及的mRNA，导致差异的基本机制及其后果，以进一步探索mRNA成熟中的这些建议方差，因为此过程中的任何失误都可能导致导致疾病的错误。总体而言，对我们遗传材料表达期间任何其他监管步骤的更详细的基本知识将为我们提供对基因调节的更好的基本了解，以及某些疾病可能会发展和未来治疗的潜在新入​​口点。