Genetic dissection of the NADPH metabolic network in drosophila

果蝇 NADPH 代谢网络的遗传解剖

基本信息

批准号：
RGPIN-2018-05551
负责人：
Merritt, Thomas
金额：
$ 3.64万
依托单位：
Laurentian University of Sudbury
依托单位国家：
加拿大
项目类别：
Discovery Grants Program - Individual
财政年份：
2020
资助国家：
加拿大
起止时间：
2020-01-01 至 2021-12-31
项目状态：
已结题

来源：
https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=712689
关键词：
Genetic dissection NADPH metabolic network

项目摘要

All organisms, from microbes to flies to people, have physical characteristics, their phenotypes, that are in part a function of their genetic material, their genotype. These phenotypes are what distinguish one organism from the next and can be as innocuous as eye color or as biologically crucial as basic metabolism or response to a particular drug. Modern science is very good at determining genotype, reading an organism's genetic material, but still working on connecting genotype to phenotype. My research seeks to better explain this connection in biological networks using the interacting genes and proteins of a metabolic network as a model system. The Long Term Goal of my research program is to describe the path between genomic molecular diversity and complex biological phenotypes, and focus on metabolic networks to understand this connection. Specifically, we quantify the genetic and metabolic interactions that regulate a network of enzymes that reduce and oxidize the metabolic cofactor NADP(H) to define and understand biological networks in general. Short-term Aims: I have two short term Aims for this granting period: 1) Define the metabolic and biochemical effects (phenotype) of variation across the NADP(H) network in Drosophila melanogaster 2) Identify novel factors (genes) that contribute to this NADP(H) network Over the 5 years of this grant, these two Aims will allow us to understand the network as a whole and provide fundamental knowledge of metabolic networks in general.

所有生物体，从微生物到苍蝇再到人类，都具有物理特征，即它们的表型，这在一定程度上是其遗传物质（即基因型）的功能。这些表型是将一种生物体与另一种生物体区分开来的，它们可以像眼睛颜色一样无害，也可以像基本代谢或对特定药物的反应一样具有生物学重要性。现代科学非常擅长确定基因型、读取生物体的遗传物质，但仍在致力于将基因型与表型联系起来。我的研究旨在使用代谢网络中相互作用的基因和蛋白质作为模型系统，更好地解释生物网络中的这种联系。我的研究计划的长期目标是描述基因组分子多样性和复杂生物表型之间的路径，并关注代谢网络以了解这种联系。具体来说，我们量化了调节还原和氧化代谢辅因子 NADP(H) 的酶网络的遗传和代谢相互作用，以定义和理解一般生物网络。短期目标：我在此授予期间有两个短期目标： 1) 定义果蝇 NADP(H) 网络变异的代谢和生化效应（表型） 2) 识别有助于该 NADP(H) 网络的新因素（基因）在这笔资助的 5 年里，这两个目标将使我们能够了解整个网络，并提供一般代谢网络的基础知识。