COVID-19 and targeting SARS-CoV-2 virus: Direct and RT-PCR sequences from wastewater collected from treatment plants (Sudbury - Ontario)

COVID-19 和针对 SARS-CoV-2 病毒：从处理厂收集的废水的直接序列和 RT-PCR 序列（安大略省萨德伯里）

• 批准号: 555122-2020
• 负责人: Merritt, Thomas
• 金额: $ 3.64万
• 依托单位: Laurentian University of Sudbury
• 依托单位国家: 加拿大
• 项目类别: Alliance Grants
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=705763
• 关键词: COVID; 19; targeting; SARS; CoV

We need to be able to rapidly and efficiently determine the presence and prevalence of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in our communities and how this presence responds to the social and medical interventions. In many ways, the raw sewage of our cities is a fantastic genetic gold mine, particularly for the pathogens that collect there. We are planning to mine this genetic resource to detect and evaluate the presence of SARS-CoV-2 using a new genetic sequencing technology. This new technology can be applied to sequence the virus genome either directly or through specific variable genetic regions using different approaches. We propose to use these two methodologies to identify and quantify the presence of the pandemic virus in the sewage collection areas. This information will be used as to estimate the virus' spread and to following changes in the outbreak through time. These results will aid local government in making informed, evidence-based, decisions to help slow and control of the pandemic. Once the protocols and relationships are well established, we will be able to scale this project quickly and extend it to different geographic areas and populations. This project is the result of a partnership between Health Sciences North, Laurentian University and the City of Greater Sudbury established to combat the pandemic.

我们需要能够快速有效地确定社区中严重急性呼吸系统综合症冠状病毒 2 (SARS-CoV-2) 的存在和流行情况，以及这种存在对社会和医疗干预措施的反应。 从许多方面来说，我们城市的原始污水是一座奇妙的基因金矿，特别是对于聚集在那里的病原体而言。我们计划利用新的基因测序技术来挖掘这种遗传资源，以检测和评估 SARS-CoV-2 的存在。这项新技术可用于直接或使用不同方法通过特定可变遗传区域对病毒基因组进行测序。我们建议使用这两种方法来识别和量化污水收集区中大流行病毒的存在。 这些信息将用于估计病毒的传播以及跟踪疫情随时间的变化。这些结果将帮助地方政府做出明智、基于证据的决策，以帮助减缓和控制疫情。一旦协议和关系建立良好，我们将能够快速扩展该项目并将其扩展到不同的地理区域和人群。该项目是北方健康科学学院、劳伦森大学和大萨德伯里市为抗击这一流行病而建立的合作伙伴关系的成果。