Development of functionalized magnetic nanoparticles to isolate and detect bacteria

开发用于分离和检测细菌的功能化磁性纳米颗粒

• 批准号: 557077-2020
• 负责人: Puri, Ishwar
• 金额: $ 2.19万
• 依托单位: McMaster University
• 依托单位国家: 加拿大
• 项目类别: Alliance Grants
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=706668
• 关键词: Development; functionalized; magnetic; nanoparticles; isolate

Detection of bacteria, such as E. coli, Legionella and Salmonella is a global problem important for the management of water bodies and agricultural farms. Municipalities and other government agencies, and private enterprises and individuals seek water quality information to make public health and policy, and business decision. For instance, such information might have prevented the worst-ever outbreak of E. coli contamination in Bruce County, Ont. in 2010 when 2,300 people fell ill and seven died (Source: CBC News, Canada). There is no current solution available that detects microbial contaminants in the water supply at the point of site. BioInk labs is partnering with the academic team to address this problem and develop a point of site test that detects bacteria in real time. Specifically, the project will develop a technology that uses magnetic nanoparticles coated with capture agents to target bacteria present in a water sample and subsequently isolate the pathogens to detect the ATP present in these bacteria. With this detection method, a color change of the sample will affirm that the target bacteria are present in it. Upon completion, the project will provide a real-world technology by incorporating the ATP sensor into a viable sensor and allow the company to create a solution for liquid borne bacteria for the global market. Municipalities, wastewater treatment facilities, government agencies, businesses, and individual users will be able to use the product to directly assess water quality in their communities or private wells in real time.

大肠杆菌、军团菌和沙门氏菌等细菌的检测是一个全球性问题，对于水体和农场的管理非常重要。市政当局和其他政府机构以及私营企业和个人寻求水质信息以做出公共卫生、政策和商业决策。例如，此类信息可能可以阻止安大略省布鲁斯县有史以来最严重的大肠杆菌污染爆发。 2010 年，有 2,300 人患病，其中 7 人死亡（来源：加拿大 CBC 新闻）。目前还没有可用的解决方案来检测现场供水中的微生物污染物。 BioInk 实验室正在与学术团队合作解决这个问题，并开发一种实时检测细菌的现场测试。具体来说，该项目将开发一种技术，使用涂有捕获剂的磁性纳米颗粒来靶向水样中存在的细菌，然后分离病原体以检测这些细菌中存在的 ATP。通过这种检测方法，样品的颜色变化将确认其中存在目标细菌。完成后，该项目将通过将 ATP 传感器整合到可行的传感器中来提供现实世界的技术，并使该公司能够为全球市场的液体传播细菌创建解决方案。市政当局、废水处理设施、政府机构、企业和个人用户将能够使用该产品直接实时评估其社区或私人水井的水质。