Evaluating the persistence of SARS-CoV-2 (COVID-19) in the urban water cycle

评估 SARS-CoV-2 (COVID-19) 在城市水循环中的持久性

• 批准号: 552125-2020
• 负责人: McKay, Robert
• 金额: $ 3.64万
• 依托单位: University of Windsor
• 依托单位国家: 加拿大
• 项目类别: Alliance Grants
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=703433
• 关键词: Evaluating; persistence; SARS; CoV; COVID

Novel coronavirus disease 2019 (COVID-19) is an acute respiratory disease which first came to the attention of the World Health Organization in early 2020. The pathogen responsible for COVID-19 is SARS-CoV-2, a member of the coronavirus family. Recent research from three continents has demonstrated the presence of SARS-CoV-2 in sewage from wastewater treatment plants (WWTPs) consistent with the virus being shed in feces. Based on this evidence, we hypothesize that SARS-CoV-2 will be detected in the influent of municipal WWTPs in Canada. While it is generally assumed that conventional wastewater treatment effectively reduces viral load, the efficacy of treatment in removing SARS-CoV-2 is not known. Even if wastewater treatment is successful, many municipalities experience plant bypass events whereby untreated or partially treated sewage is discharged directly into receiving waters. In these cases, it is not known how long SARS-CoV-2 will persist in the environment if released from WWTP effluent. Working with our partners at the Town of Lakeshore, ON and the Town of Amherstburg, ON, we will address two research objectives: 1) to assess trends/changes in occurrence of community infection from sewage surveillance 2) to evaluate persistence of SARS-CoV-2 in wastewater effluent: a. using surrogate phage b. during a bypass event Information gained from this study will inform municipalities on best practices in wastewater treatment to address the safety of our water supply and build capability to address future pandemics. Data on viral load in influent will provide a proxy to assess trends in community infections in lieu of widescale testing of individuals and will provide early warning to a possible 'second wave' of infection. The partnership also offers opportunities to test hypotheses related to persistence of SARS-CoV-2 on occasions when it is discharged to the environment, in this case Lake St. Clair and the binational Detroit River Great Lakes Area of Concern.

2019 新型冠状病毒病 (COVID-19) 是一种急性呼吸道疾病，于 2020 年初首次引起世界卫生组织的关注。导致 COVID-19 的病原体是 SARS-CoV-2，它是冠状病毒家族的成员。来自三大洲的最新研究表明，废水处理厂 (WWTP) 污水中存在 SARS-CoV-2，这与粪便中传播的病毒一致。基于这一证据，我们假设将在加拿大市政污水处理厂的进水中检测到 SARS-CoV-2。虽然人们普遍认为传统废水处理可有效降低病毒载量，但处理去除 SARS-CoV-2 的功效尚不清楚。即使废水处理成功，许多城市也会经历工厂旁路事件，未经处理或部分处理的污水直接排入受纳水域。在这些情况下，尚不清楚 SARS-CoV-2 从污水处理厂废水中释放后会在环境中持续存在多久。我们将与安大略省莱克肖尔镇和阿默斯特堡镇的合作伙伴合作，实现两个研究目标： 1) 通过污水监测评估社区感染发生的趋势/变化 2) 评估废水中 SARS-CoV-2 的持久性： 一个。使用替代噬菌体 b.在旁路事件期间 从这项研究中获得的信息将为市政当局提供废水处理的最佳实践，以解决供水安全问题并建立应对未来流行病的能力。进水病毒载量数据将提供评估社区感染趋势的指标，以代替对个人进行大规模检测，并将为可能出现的“第二波”感染提供早期预警。该合作伙伴关系还提供了测试与 SARS-CoV-2 排放到环境中时的持久性相关的假设的机会，在本例中是圣克莱尔湖和两国底特律河五大湖关注区。