Persistence of SARS-CoV-2 in Wastewater

废水中 SARS-CoV-2 的持续存在

• 批准号: 10159715
• 负责人: Samendra P Sherchan
• 金额: $ 9万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-01 至 2022-03-03
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10159715
• 关键词: 2019-nCoV; Address; Arizona; COVID-19; China; Chloramines; Chlorine; Containment; Contracts; Controlled Study; Coronavirus; Development; Disease; Disinfectants; Disinfection; Ensure; Enterocytes; Evaluation; Family; Feces; Federal Government; Goals; Health; Human; Individual; Infrastructure; Knowledge; Laboratories; Light; Local Government; Locales; Louisiana; Monitor; Names; Oral; Persons; Plants; Prevention; Public Health; RNA Viruses; Recovery; Resources; Respiratory Signs and Symptoms; Respiratory System; Risk; Risk Assessment; Safety; Sewage; System; Time; Vaccination; Virion; Virus; Water; Work; World Health Organization; chlorine dioxide; economic impact; experimental study; field survey; human coronavirus; infection risk; member; microbial; novel; pandemic disease; transmission process

Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2), member of the Coronavirus family, has recently emerged from Wuhan, China. The World Health Organization (WHO) announced an official name of the disease [coronavirus disease 2019 (COVID-19)] and classified it as a global pandemic. Though the virus is primarily spread through person-to-person contact and causes respiratory tract illnesses; some recent studies have suggested the possibility for fecal-oral transmission and detected the RNA virus in the feces of infected individuals, even after respiratory symptoms have subsided. A recent study confirmed that the virus is also capable of infecting human gut enterocytes (Lamers et al., 2020). However, the potential transmission of SARS- CoV-2 via wastewater is currently unknown. Therefore, the proposed study here will facilitate a better understanding of persistence and disinfection of SARS-CoV-2 in wastewater. First, we will use laboratory- controlled studies examining the survivability of the virus in wastewater. We will spike the SARS-CoV-2 in wastewater and monitor infectivity over time. Second, we will use chlorine disinfectants to determine the inactivation of SARS-CoV-2 in wastewater. Last, we will conduct a field survey to investigate the fate of the virus in wastewater treatment systems from different locales. The proposed work will provide information to state and federal regulatory agencies that can be used to develop guidance for prevention of COVID-19. The project will also aid in evaluation and development of best disinfection approaches and quantitative microbial risk assessment (QMRA). The findings will be invaluable to water resource recovery facilities for better management against potential exposure risk and ensuring worker health and safety.

严重急性呼吸系统综合症冠状病毒-2 (SARS-CoV-2) 是冠状病毒家族的成员，已 最近从中国武汉出现。世界卫生组织（WHO）公布了该组织的正式名称 疾病 [2019 年冠状病毒病 (COVID-19)] 并将其归类为全球大流行病。虽然病毒是 主要通过人与人之间的接触传播并引起呼吸道疾病；最近的一些研究 提出了粪口传播的可能性，并在感染者的粪便中检测到了 RNA 病毒 个人，即使呼吸道症状已经消退。最近的一项研究证实，该病毒还 能够感染人类肠道肠细胞 (Lamers et al., 2020)。然而，SARS 的潜在传播 目前未知通过废水传播的 CoV-2。因此，这里提出的研究将有助于更好地 了解废水中 SARS-CoV-2 的持久性和消毒作用。首先，我们将使用实验室- 检查废水中病毒的生存能力的对照研究。我们将在 废水并随着时间的推移监测传染性。其次，我们将使用含氯消毒剂来确定 废水中 SARS-CoV-2 的灭活。最后，我们将进行实地调查，调查病毒的命运 来自不同地点的废水处理系统。拟议的工作将为国家和 联邦监管机构可用于制定预防 COVID-19 的指南。该项目将 还有助于评估和开发最佳消毒方法和定量微生物风险 评估（QMRA）。研究结果对于水资源回收设施的更好管理具有无价的价值 防范潜在的暴露风险，确保工人的健康和安全。