Back to Capacity: Replacement of a 20-year Old Non-servicable Automated Gas Chromatograph for the Determination of Greenhouse Gas Emissions from Agriculture

恢复产能：更换已使用 20 年、无法维修的自动气相色谱仪，用于测定农业温室气体排放

• 批准号: RTI-2020-00451
• 负责人: Tenuta, Mario
• 金额: $ 7.59万
• 依托单位: University of Manitoba
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=692869
• 关键词: Back; Capacity; Replacement; 20; year

Dr. Tenuta's Applied Soil Ecology Laboratory has fully utilized two automated gas chromatographs since 2005 for the determination of the emissions of the greenhouse gases nitrous oxide, methane, and carbon dioxide from agricultural soils and manure. Recently, the oldest of the instruments purchased in 1999 became inoperable and past its serviceable lifespan. Dr. Tenuta's lab urgently requires replacement of the system to analyze a backlog of samples collected this year and to start new funded projects dependent on the collection of even a greater number of samples next year. Other instrumentation is not available at the University, and commercial services are unavailable. The proposed instrument is similar in configuration to the one it replaces as the needs of gas analysis and sample handling have not changed. The instrument is configured for high throughput with an autosampler and capable of analysis of 250 samples per day of air-tight sample vials. The three most important greenhouse gases for agriculture are analyzed simultaneously as the instrument is equipped with detectors for each gas. The lab strives to Better Soil Nitrogen Use Towards Improved Crop and Environmental Health. A keystone of the research is the comparison of farmer management practices that reduce greenhouse gas emissions. The primary method employed to determine emissions is the collection of gases accumulated within chambers on the soil or manure pile surfaces. The gas chromatograph is ideally suited to high throughput analysis of the gases stored in glass vials. The instrument is also central to the training of students and other highly qualified personnel in the operation, analysis and quality control of gas concentration results. The operation and results from the instrument bring full circle the training of HQP from hypothesis generation, setup, and maintenance of field study sites and treatments, gas collection, gas chromatograph operation and analysis, processing of gas results to emission rates, statistical analyses, and conclusion to hypotheses and communication of findings. A per sample analysis fee for each of the lab's samples and those of his collaborators supports the operational readiness of the instrument as well as training in the use of the instrument and its results. An anticipated eight graduate and nine undergraduate students and two post-doctoral fellows will benefit from the instrument being central to their training in Dr. Tenuta's lab. He is highly committed to fostering the removal of barriers facing women and minorities in the soil sciences. This includes providing training in the use of the instrument regardless of a student's gender, race, and beliefs. Training on the instrument is tailored to the individual's confidence and experience in the previous operation of scientific equipment bringing all to a level of familiarity and sureness to benefit their emerging careers.

Tenuta 博士的应用土壤生态实验室自 2005 年起充分利用两台自动气相色谱仪来测定农业土壤和粪便中温室气体一氧化二氮、甲烷和二氧化碳的排放量。最近，1999 年购买的最旧的仪器无法使用并超过了使用寿命。 Tenuta 博士的实验室迫切需要更换系统来分析今年收集的积压样本，并根据明年收集的更多样本启动新的资助项目。大学不提供其他仪器，也不提供商业服务。拟议的仪器在配置上与它所取代的仪器相似，因为气体分析和样品处理的需求没有改变。该仪器配备自动进样器，可实现高通量，每天能够分析 250 个气密样品瓶样品。由于仪器配备了每种气体的探测器，因此可以同时分析农业中三种最重要的温室气体。该实验室致力于更好地利用土壤氮，以改善作物和环境健康。该研究的一个重点是比较减少温室气体排放的农民管理实践。确定排放量的主要方法是收集土壤或粪肥堆表面的室内积聚的气体。该气相色谱仪非常适合对玻璃瓶中储存的气体进行高通量分析。该仪器也是对学生和其他高素质人员进行气体浓度结果操作、分析和质量控制培训的核心。仪器的操作和结果完整地完成了 HQP 的培训，从假设生成、现场研究地点和处理的设置和维护、气体收集、气相色谱仪操作和分析、气体结果处理到排放率、统计分析和假设的结论和研究结果的交流。实验室的每个样品及其合作者的样品分析费用支持仪器的操作准备以及仪器使用及其结果的培训。预计八名研究生、九名本科生和两名博士后研究员将受益于该仪器，该仪器是他们在 Tenuta 博士实验室培训的核心。他高度致力于消除妇女和少数族裔在土壤科学领域面临的障碍。这包括提供乐器使用培训，无论学生的性别、种族和信仰如何。仪器培训根据个人之前操作科学设备的信心和经验量身定制，使所有人达到熟悉和确定的水平，从而有利于他们的新兴职业。