Investigating bacterial virulence of aquatic pathogens using multiple host-pathogen models

使用多种宿主-病原体模型研究水生病原体的细菌毒力

• 批准号: RGPIN-2019-04444
• 负责人: Charette, Steve
• 金额: $ 3.06万
• 依托单位: Université Laval
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=688488
• 关键词: Investigating; bacterial; virulence; aquatic; pathogens

Virulence is defined as the ability of a bacterial pathogen to injure a host by producing factors that are essential for the pathogen to invade, multiply and thrive in the host. There is an ongoing research effort to identify and functionally describe these factors in order to understand the behavior of bacterial pathogens. This research is also crucial for the development of judicious treatments that can be used as alternatives to antibiotics which are less and less effective against a wide variety of pathogens infecting humans, but also animals. For example, the aquaculture is afflicted worldwide by the emergence of antibiotic multi-resistant pathogens.******The long-term objective of my research program is to identify the molecular basis of the virulence factors produced by aquatic pathogens as well as ways to inhibit or modulate them in order to prevent or treat bacterial infections. ******Over the next five years, my research program will continue to concentrate on the study of Aeromonas salmonicida, a major fish pathogen. We will continue to explore the involvement of the numerous mobile genetic elements in the instability of its genome leading to loss of virulence factors in response to stressful conditions. We will also initiate research on the interaction of A. salmonicida with protozoa and fish in a new AQuatic Containment level 2 (AQC2) in vivo facilities, opening the door to experiments not feasible in the past.******My proposed research program will provide a better understanding of the virulence of A. salmonicida and of pathogens in general including its interactions with multiple hosts. Based on our ongoing effort to understand the pathogenic basis of A. salmonicida and the objectives set out in this proposal, we are confident that concrete treatments against this bacterium will be possible on a relative short term.

毒力被定义为细菌病原体通过产生对病原体入侵，繁殖和繁殖在宿主中至关重要的因素来损害宿主的能力。正在进行的研究工作旨在识别和在功能上描述这些因素，以了解细菌病原体的行为。这项研究对于开发明智的治疗方法也至关重要，这些疗法可以用作抗生素的替代方法，这些抗生素越来越有效地针对感染人类和动物的各种病原体。例如，由于我的研究计划的长期目标是确定水生病原体产生的毒力因子的分子基础，以及用于预防或治疗细菌感染的方法，因此，通过抗生素多耐病病原体的出现使水产养殖受到折磨。******。 ******在接下来的五年中，我的研究计划将继续专注于对主要的鱼类病原体的Aeromonas Salmonicida研究。我们将继续探索众多移动遗传因素在其基因组的不稳定性中的参与，从而导致毒力因素因压力条件而丧失。我们还将在新的水生遏制2（AQC2）的体内设施中进行有关萨尔霉菌与原生动物和鱼类的相互作用的研究，为过去不可行的实验打开了我建议的研究计划。基于我们持续不断地了解salmonicida的致病基础以及该提案中提出的目标，我们相信在相对短期内可以使用针对该细菌的具体治疗方法。